Effect of nitric oxide on boar sperm motility, membrane integrity, and acrosomal status during semen storage

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F18%3A76548" target="_blank" >RIV/60460709:41210/18:76548 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.24425/119024" target="_blank" >http://dx.doi.org/10.24425/119024</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.24425/119024" target="_blank" >10.24425/119024</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of nitric oxide on boar sperm motility, membrane integrity, and acrosomal status during semen storage

Popis výsledku v původním jazyce

Nitric oxide (NO) is a major gasotransmitter involved in several physiological processes of male reproduction. There is, nevertheless, little information concerning the role of NO during semen storage. The aim of this study was to evaluate the effect of NO on boar semen stored at 17 C for 72 h. For this purporse, sperm samples were treated with 0,625, 1,25, 2,5, 5, and 10 mM aminoguanidine (AG) or N omega Nitro L arginine methyl ester hydrochloride (L NAME), a selective and nonselective NO synthase (NOS) inhibitor, respectively. Moreover, sodium nitroprusside (SNP), a NO donor, was used at the dose of 18,75, 37,5, 75, and 150 microM. Sperm motility, membrane integrity, and acrosomal status were evaluated at 0, 4, 24, 48, and 72 h of semen storage. A significant increase of the amplitude of lateral sperm head displacement (ALH), and both curvilinear and straight-line velocity (VCL and VSL, respectively) was observed at 72 h of semen storage in samples treated with 0,625 mM AG, probably beca

Název v anglickém jazyce

Effect of nitric oxide on boar sperm motility, membrane integrity, and acrosomal status during semen storage

Popis výsledku anglicky

Nitric oxide (NO) is a major gasotransmitter involved in several physiological processes of male reproduction. There is, nevertheless, little information concerning the role of NO during semen storage. The aim of this study was to evaluate the effect of NO on boar semen stored at 17 C for 72 h. For this purporse, sperm samples were treated with 0,625, 1,25, 2,5, 5, and 10 mM aminoguanidine (AG) or N omega Nitro L arginine methyl ester hydrochloride (L NAME), a selective and nonselective NO synthase (NOS) inhibitor, respectively. Moreover, sodium nitroprusside (SNP), a NO donor, was used at the dose of 18,75, 37,5, 75, and 150 microM. Sperm motility, membrane integrity, and acrosomal status were evaluated at 0, 4, 24, 48, and 72 h of semen storage. A significant increase of the amplitude of lateral sperm head displacement (ALH), and both curvilinear and straight-line velocity (VCL and VSL, respectively) was observed at 72 h of semen storage in samples treated with 0,625 mM AG, probably beca

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

POLISH JOURNAL OF VETERINARY SCIENCES

ISSN

1505-1773

e-ISSN

—

Svazek periodika

21

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

10

Strana od-do

73-82

Kód UT WoS článku

000432043500009

EID výsledku v databázi Scopus

2-s2.0-85046774759