Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027014%3A_____%2F23%3A10005830" target="_blank" >RIV/00027014:_____/23:10005830 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/23:N0000225

Výsledek na webu

<a href="https://www.mdpi.com/2076-2615/13/13/2144" target="_blank" >https://www.mdpi.com/2076-2615/13/13/2144</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/ani13132144" target="_blank" >10.3390/ani13132144</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Popis výsledku v původním jazyce

Simple Summary Caseous lymphadenitis is a contagious bacterial disease that affects both domestic and wild animals. It has been studied by scientists since the 19th century. Thanks to new high-throughput RNA sequencing techniques, we can now better understand how the immune system responds to this disease, evaluate individual resistance levels, and identify disease biomarkers. This knowledge can help prevent the spread of incurable diseases, reduce economic losses, and breed farm animals with greater bacterial resistance. In this study, we explore the potential role of immune genes in the fight against disease in ewes from an ordinary farm. The ovine genome reflected host-pathogen interactions by promoting transcriptional changes in innate and acquired immunity in diseased sheep. Furthermore, in exposed sheep, innate immunity increased over the adaptive response. Caseous lymphadenitis (CL) is a chronic contagious disease that affects small ruminants and is characterized by the formation of pyogranulomas in lymph nodes and other organs. However, the pathogenesis of this disease and the response of the host genome to infection are not yet fully understood. This study aimed to investigate the whole blood transcriptome and evaluate differential gene expression during the later stages of CL in naturally infected ewes. The study included diseased, serologically positive (EP), exposed, serologically negative (EN) ewes from the same infected flock and healthy ewes (CN) from a different flock. RNA sequencing was performed using the Illumina NextSeq system, and differential gene expression was estimated using DESeq2 and Edge R approaches. The analysis identified 191 annotated differentially expressed genes (DEGs) in the EP group (102 upregulated and 89 downregulated) and 256 DEGs in the EN group (106 upregulated and 150 downregulated) compared to the CN group. Numerous immunoregulatory interactions between lymphoid and nonlymphoid cells were influenced in both EP and EN ewes. Immune DEGs were preferentially assigned to antigen presentation through the MHC complex, T lymphocyte-mediated immunity, and extracellular matrix interactions. Furthermore, the EP group showed altered regulation of cytokine and chemokine signaling and activation and recombination of B-cell receptors. Conversely, NF-kappa B signaling, apoptosis, and stress response were the main processes influenced in the EN group. In addition, statistically significant enrichment of the essential immune pathways of binding and uptake of ligands by scavenger receptors in EP and p53 signaling in the EN group was found. In conclusion, this study provides new insights into the disease course and host-pathogen interaction in naturally CL-infected sheep by investigating the blood transcriptome.

Název v anglickém jazyce

Comparative Characterization of Immune Response in Sheep with Caseous Lymphadenitis through Analysis of the Whole Blood Transcriptome

Popis výsledku anglicky

Simple Summary Caseous lymphadenitis is a contagious bacterial disease that affects both domestic and wild animals. It has been studied by scientists since the 19th century. Thanks to new high-throughput RNA sequencing techniques, we can now better understand how the immune system responds to this disease, evaluate individual resistance levels, and identify disease biomarkers. This knowledge can help prevent the spread of incurable diseases, reduce economic losses, and breed farm animals with greater bacterial resistance. In this study, we explore the potential role of immune genes in the fight against disease in ewes from an ordinary farm. The ovine genome reflected host-pathogen interactions by promoting transcriptional changes in innate and acquired immunity in diseased sheep. Furthermore, in exposed sheep, innate immunity increased over the adaptive response. Caseous lymphadenitis (CL) is a chronic contagious disease that affects small ruminants and is characterized by the formation of pyogranulomas in lymph nodes and other organs. However, the pathogenesis of this disease and the response of the host genome to infection are not yet fully understood. This study aimed to investigate the whole blood transcriptome and evaluate differential gene expression during the later stages of CL in naturally infected ewes. The study included diseased, serologically positive (EP), exposed, serologically negative (EN) ewes from the same infected flock and healthy ewes (CN) from a different flock. RNA sequencing was performed using the Illumina NextSeq system, and differential gene expression was estimated using DESeq2 and Edge R approaches. The analysis identified 191 annotated differentially expressed genes (DEGs) in the EP group (102 upregulated and 89 downregulated) and 256 DEGs in the EN group (106 upregulated and 150 downregulated) compared to the CN group. Numerous immunoregulatory interactions between lymphoid and nonlymphoid cells were influenced in both EP and EN ewes. Immune DEGs were preferentially assigned to antigen presentation through the MHC complex, T lymphocyte-mediated immunity, and extracellular matrix interactions. Furthermore, the EP group showed altered regulation of cytokine and chemokine signaling and activation and recombination of B-cell receptors. Conversely, NF-kappa B signaling, apoptosis, and stress response were the main processes influenced in the EN group. In addition, statistically significant enrichment of the essential immune pathways of binding and uptake of ligands by scavenger receptors in EP and p53 signaling in the EN group was found. In conclusion, this study provides new insights into the disease course and host-pathogen interaction in naturally CL-infected sheep by investigating the blood transcriptome.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40203 - Husbandry

Projekt

<a href="/cs/project/QK1910082" target="_blank" >QK1910082: Řešení problematiky výskytu bakteriálních, protozoárních a virových zoonotických agens v chovech malých přežvýkavců</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Animals

ISSN

2076-2615

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

2144

Kód UT WoS článku

001028476800001

EID výsledku v databázi Scopus

2-s2.0-85165045892