Influence of high pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F09%3A00000057" target="_blank" >RIV/00027022:_____/09:00000057 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of high pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests

Popis výsledku v původním jazyce

The aim of our study was to detect the influence of high pressure treatment (HPT) on allergenicity of recombinant allergen rDau c1 and carrot juice using in vitro and in vivo tests. The buffer solution of recombinant main carrot allergen r Dau c1 for basophil activation test (BAT) and Western blot (WB) was used. Dau c1 was pre-treated by pressure 500 MPa for 10 minutes and different temperatures (30, 40, 50°C) and pressure from 400 to 550 MPa for 3 and 10 minutes. Neither the HPT from 400 to 550 MPa for3 and 10 minutes nor the HPT at 500 MPa for 10 minutes and temperature 30, 40 and 50°C had the influence on basophil activation by rDau c1. Serum samples of birch pollen allergic patients reacted in WB with solution of rDau c1 treated by HP 500 MPa for10 minutes at temperature 30, 40 and 50°C. This pressure procedure did not influence the immune reactivity of rDau c1 in WB test. The structural changes of rDau c1 caused by HPT studied by circular dichroismus (CD) spectra were found.

Název v anglickém jazyce

Influence of high pressure treatment on allergenicity of rDau c1 and carrot juice demonstrated by in vitro and in vivo tests

Popis výsledku anglicky

The aim of our study was to detect the influence of high pressure treatment (HPT) on allergenicity of recombinant allergen rDau c1 and carrot juice using in vitro and in vivo tests. The buffer solution of recombinant main carrot allergen r Dau c1 for basophil activation test (BAT) and Western blot (WB) was used. Dau c1 was pre-treated by pressure 500 MPa for 10 minutes and different temperatures (30, 40, 50°C) and pressure from 400 to 550 MPa for 3 and 10 minutes. Neither the HPT from 400 to 550 MPa for3 and 10 minutes nor the HPT at 500 MPa for 10 minutes and temperature 30, 40 and 50°C had the influence on basophil activation by rDau c1. Serum samples of birch pollen allergic patients reacted in WB with solution of rDau c1 treated by HP 500 MPa for10 minutes at temperature 30, 40 and 50°C. This pressure procedure did not influence the immune reactivity of rDau c1 in WB test. The structural changes of rDau c1 caused by HPT studied by circular dichroismus (CD) spectra were found.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/2B06139" target="_blank" >2B06139: Inaktivace alergenů v potravinách pomocí vysokotlaké pasterace</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

High Pressure Research

ISSN

1477-2299

e-ISSN

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Svazek periodika

29

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

000274675700043

EID výsledku v databázi Scopus

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