Multiplex immunoline test for food allergens detection. Poster a sborník 15th International Nutrition and Diagnostics Conference (INDC 2015) Prague, Czech Republic, October 5 - 8, 2015, ISBN978-80-7395-901-2

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F15%3A%230000030" target="_blank" >RIV/00027022:_____/15:#0000030 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Multiplex immunoline test for food allergens detection. Poster a sborník 15th International Nutrition and Diagnostics Conference (INDC 2015) Prague, Czech Republic, October 5 - 8, 2015, ISBN978-80-7395-901-2

Popis výsledku v původním jazyce

Some allergenic products and substances contained in food must be indicated on the label in accordance with the EU legislation (Regulation EU No 1169/2011). Cereals containing gluten, milk, eggs, nuts, peanuts, soybeans, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites belong to the group of mandatory declared allergens. The development of a highly sensitive and specific multiplex test able to detect more allergens within a single assay was aim of this work. Multiplex Immunoline test is specific against allergens Arah1 (peanut), beta-lactoglobulin (milk), casein (milk), egg white proteins (egg), tree nuts proteins from hazelnuts, walnuts, almonds, pistachios, Brazil nuts, macadamia nuts and cashew and mustard proteins from Sinapsis alba, Brassica nigra, Brassica juncea, Rape seed. The presence of food allergens can be find out within a short time, which can lead to time savings for food producers and trade in food commodities. Multiplex Immunoline test is based on the binding of antibodies immobilized on the solid phase to an antigens extracted from food samples. The detection is carried out by the biotinylated specific antibodies, streptavidin - alkaline phosphatase and a BCIP/NBT colorimetrics membrane AP substrate. The different categories of commercial food products were tested for the Immunoline validation and the stability and reproducibility tests were performed.

Název v anglickém jazyce

Multiplex immunoline test for food allergens detection. Poster a sborník 15th International Nutrition and Diagnostics Conference (INDC 2015) Prague, Czech Republic, October 5 - 8, 2015, ISBN978-80-7395-901-2

Popis výsledku anglicky

Some allergenic products and substances contained in food must be indicated on the label in accordance with the EU legislation (Regulation EU No 1169/2011). Cereals containing gluten, milk, eggs, nuts, peanuts, soybeans, fish, crustaceans, molluscs, celery, lupin, sesame, mustard and sulphites belong to the group of mandatory declared allergens. The development of a highly sensitive and specific multiplex test able to detect more allergens within a single assay was aim of this work. Multiplex Immunoline test is specific against allergens Arah1 (peanut), beta-lactoglobulin (milk), casein (milk), egg white proteins (egg), tree nuts proteins from hazelnuts, walnuts, almonds, pistachios, Brazil nuts, macadamia nuts and cashew and mustard proteins from Sinapsis alba, Brassica nigra, Brassica juncea, Rape seed. The presence of food allergens can be find out within a short time, which can lead to time savings for food producers and trade in food commodities. Multiplex Immunoline test is based on the binding of antibodies immobilized on the solid phase to an antigens extracted from food samples. The detection is carried out by the biotinylated specific antibodies, streptavidin - alkaline phosphatase and a BCIP/NBT colorimetrics membrane AP substrate. The different categories of commercial food products were tested for the Immunoline validation and the stability and reproducibility tests were performed.

Druh

O - Ostatní výsledky

CEP obor

GM - Potravinářství

OECD FORD obor

—

Projekt

<a href="/cs/project/TA03010625" target="_blank" >TA03010625: Nové materiály a technologie pro vývoj multiplexových testů pro komplexní diagnostiku ve zdravotnictví a pro detekci alergenů v potravinách</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů