International interlaboratory study on TaqMan real-time polymerase chain reaction authentication of black seabream (Spondyliosoma cantharus)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F18%3AN0000008" target="_blank" >RIV/00027022:_____/18:N0000008 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/18:10390775 RIV/60461373:22330/18:43917524 RIV/75010330:_____/18:00012196

Výsledek na webu

<a href="http://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=57&article=2084" target="_blank" >http://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=57&article=2084</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

International interlaboratory study on TaqMan real-time polymerase chain reaction authentication of black seabream (Spondyliosoma cantharus)

Popis výsledku v původním jazyce

An interlaboratory study of five laboratories testing real-time polymerase chain reaction (PCR) with TaqMan probe detection of black seabream (Spondyliosoma cantharus) fish is presented in this work. The method is oriented to the intron of the nuclear gene encoding the main protein of the musculature of the fish, parvalbumin. This feature distinguishes the employed approach from those based on mitochondrial genes. Here, the intron is flanked by exon stretches highly conserved among species. This provides a unique advantage when a new fish species emerges as a commodity on the market: Species-versatile degenerate primers can be easily designed on these conserved exon stretches. Therefore, the initial uncertainty of the species-specific sequence of the intron can, in such case, be bypassed during the adoption of the method to this particular new species, because the amplicon obtained at this pilot stage provides the sequence of the intron itself. DNA isolates from eight exemplars of S. cantharus and from nineteen other fish species, the latter being used as negative controls, were tested in this study by participating laboratories. The readouts in qualitative assessment were 100% accurate. The quantitative results provided an average value and variation among samples representing particular exemplars of S. cantharus.

Název v anglickém jazyce

International interlaboratory study on TaqMan real-time polymerase chain reaction authentication of black seabream (Spondyliosoma cantharus)

Popis výsledku anglicky

An interlaboratory study of five laboratories testing real-time polymerase chain reaction (PCR) with TaqMan probe detection of black seabream (Spondyliosoma cantharus) fish is presented in this work. The method is oriented to the intron of the nuclear gene encoding the main protein of the musculature of the fish, parvalbumin. This feature distinguishes the employed approach from those based on mitochondrial genes. Here, the intron is flanked by exon stretches highly conserved among species. This provides a unique advantage when a new fish species emerges as a commodity on the market: Species-versatile degenerate primers can be easily designed on these conserved exon stretches. Therefore, the initial uncertainty of the species-specific sequence of the intron can, in such case, be bypassed during the adoption of the method to this particular new species, because the amplicon obtained at this pilot stage provides the sequence of the intron itself. DNA isolates from eight exemplars of S. cantharus and from nineteen other fish species, the latter being used as negative controls, were tested in this study by participating laboratories. The readouts in qualitative assessment were 100% accurate. The quantitative results provided an average value and variation among samples representing particular exemplars of S. cantharus.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40103 - Fishery

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food and Nutrition Research

ISSN

1336-8672

e-ISSN

1338-4260

Svazek periodika

57

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

SK - Slovenská republika

Počet stran výsledku

11

Strana od-do

27-37

Kód UT WoS článku

000430630000003

EID výsledku v databázi Scopus

2-s2.0-85044600650