Interlaboratory identification of Black seabream (Spondyliosoma cantharus) as a model species on basis of PCR targeting the second intron of the parvalbumin gene

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F14%3A43898436" target="_blank" >RIV/60461373:22330/14:43898436 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027022:_____/14:00000492

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1111/jfq.12114/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/jfq.12114/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/jfq.12114" target="_blank" >10.1111/jfq.12114</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interlaboratory identification of Black seabream (Spondyliosoma cantharus) as a model species on basis of PCR targeting the second intron of the parvalbumin gene

Popis výsledku v původním jazyce

An end-point polymerase chain reaction (PCR) targeting the second intron in the protein-coding region of the parvalbumin gene of black seabream (Spondyliosoma cantharus) was used to identify this fish species. The reproducibility of the method was testedby a collaborative study in which four laboratories participated. Twenty-eight samples of isolated DNA from fish meat were tested in participating laboratories by the presented identification method. Nine samples were from black seabream and the remaining 19 were fish flesh from the reference panel. In parallel, six frozen samples of fish meat, where three were of black seabream and remaining three from the reference panel, were tested in the laboratories by this method after various DNA isolation procedures. The PCR-based method for species determination of black seabream presented in this study proved to be a reliable and independent on method of DNA isolation from meat among the isolation techniques used. Practical ApplicationsThis

Název v anglickém jazyce

Interlaboratory identification of Black seabream (Spondyliosoma cantharus) as a model species on basis of PCR targeting the second intron of the parvalbumin gene

Popis výsledku anglicky

An end-point polymerase chain reaction (PCR) targeting the second intron in the protein-coding region of the parvalbumin gene of black seabream (Spondyliosoma cantharus) was used to identify this fish species. The reproducibility of the method was testedby a collaborative study in which four laboratories participated. Twenty-eight samples of isolated DNA from fish meat were tested in participating laboratories by the presented identification method. Nine samples were from black seabream and the remaining 19 were fish flesh from the reference panel. In parallel, six frozen samples of fish meat, where three were of black seabream and remaining three from the reference panel, were tested in the laboratories by this method after various DNA isolation procedures. The PCR-based method for species determination of black seabream presented in this study proved to be a reliable and independent on method of DNA isolation from meat among the isolation techniques used. Practical ApplicationsThis

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Quality

ISSN

0146-9428

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

429-436

Kód UT WoS článku

000346265100005

EID výsledku v databázi Scopus

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