PCR-based fish meat authentication

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F17%3A00000677" target="_blank" >RIV/00027022:_____/17:00000677 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.crf2017.eu/crf_2017_BoA.html" target="_blank" >http://www.crf2017.eu/crf_2017_BoA.html</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PCR-based fish meat authentication

Popis výsledku v původním jazyce

The most common determination of fish species is based on morphological traits. This approach faces more and more complications as the level of processing of fish flesh into products of food industry and/or complex dishes produced in gastronomy makes morphological markers less available. Another factor preventing morphological determination is the character of marine fish trade and import of this fish into landlocked countries like the Czech Republic. These are often transported in the form of frozen blocks, also devoid of morphological characteristics. It is therefore critical to have a method for species determination independent on size, intactness or level of processing of the sample, available as a versatile tool. Towards this end, methods of molecular biology based on Polymerase Chain Reaction (PCR) appear as the best tool due to their high level of sensitivity and specificity in raw as well as technologically processed fish meat, even in the form of complex food products. The presented analysis is performed as an amplification of nuclear gene encoding important protein of fish muscles parvalbumin, also known as a major allergen connected with fish consumption. Here, within the scope of Real-Time PCR (qPCR) set of primers together with fluorescent dye-conjugated probe, both specific to second intron of protein coding region of Black seabream (Spondyliosoma cantharus) were designed and tested. Specificity of this set was validated on panel of 19 different fish species representing the most common and important fraction of the spectrum of the locally traded species, i.e. for instance Carp (Cyprinus carpio), Tench (Tinca tinca), Nile tilapia (Oreochromis niloticus) and others. Efficiency of the method was determined from calibration curve based on points of five levels of DNA concentration. Quantitative PCR presented here was proven as highly efficient and specific method for Black seabream (S. cantharus) species identification.

Název v anglickém jazyce

PCR-based fish meat authentication

Popis výsledku anglicky

The most common determination of fish species is based on morphological traits. This approach faces more and more complications as the level of processing of fish flesh into products of food industry and/or complex dishes produced in gastronomy makes morphological markers less available. Another factor preventing morphological determination is the character of marine fish trade and import of this fish into landlocked countries like the Czech Republic. These are often transported in the form of frozen blocks, also devoid of morphological characteristics. It is therefore critical to have a method for species determination independent on size, intactness or level of processing of the sample, available as a versatile tool. Towards this end, methods of molecular biology based on Polymerase Chain Reaction (PCR) appear as the best tool due to their high level of sensitivity and specificity in raw as well as technologically processed fish meat, even in the form of complex food products. The presented analysis is performed as an amplification of nuclear gene encoding important protein of fish muscles parvalbumin, also known as a major allergen connected with fish consumption. Here, within the scope of Real-Time PCR (qPCR) set of primers together with fluorescent dye-conjugated probe, both specific to second intron of protein coding region of Black seabream (Spondyliosoma cantharus) were designed and tested. Specificity of this set was validated on panel of 19 different fish species representing the most common and important fraction of the spectrum of the locally traded species, i.e. for instance Carp (Cyprinus carpio), Tench (Tinca tinca), Nile tilapia (Oreochromis niloticus) and others. Efficiency of the method was determined from calibration curve based on points of five levels of DNA concentration. Quantitative PCR presented here was proven as highly efficient and specific method for Black seabream (S. cantharus) species identification.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů