Fish species identification by pcr using parvalbumin gene as a platform

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F19%3A43920124" target="_blank" >RIV/60461373:22330/19:43920124 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027022:_____/20:999

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fish species identification by pcr using parvalbumin gene as a platform

Popis výsledku v původním jazyce

Food fraud is a significant and growing problem. The increase in international trade, increasing global consumption of fish and different level of supplies and demands for some species, have led to many cases of economic frauds. In this case one type of fish product is illegally replaced by another. This is big economical problem, because mislabeling can result as fraudulent substitution of meat with high value with some less expensive fish. Moreover, proper labeling is also important in terms of the impact on health, certain people can consume only specific fishes, because of allergic sensitivity. The most common determination of fish species is based on morphological traits. This approach faces more and more complications as the level of processing fish flesh into products of food industry and/or complex dishes in gastronomy makes morphological markers less available. Methods based on the polymerase chain reaction (PCR) are the most spread for control purposes, due to the high level of sensitivity and specificity. The presented analysis is performed as an amplification of nuclear gene encoding important protein of fish muscles parvalbumin, also known as a major fish allergen. Conventional PCR method for the differentiation of the following fish species were developed: black seabream (Sponyliosoma cantharus), Atlantic mackerel (Scomber scombrus), iridescent shark (Pangasianodon hypophthalmus) and angler fish (Lophius piscatorius). Specificity was verified on panel of 19 different fish species. This method can be employed as a routine tool for species origin determination irrespectively of morphological traits.

Název v anglickém jazyce

Fish species identification by pcr using parvalbumin gene as a platform

Popis výsledku anglicky

Food fraud is a significant and growing problem. The increase in international trade, increasing global consumption of fish and different level of supplies and demands for some species, have led to many cases of economic frauds. In this case one type of fish product is illegally replaced by another. This is big economical problem, because mislabeling can result as fraudulent substitution of meat with high value with some less expensive fish. Moreover, proper labeling is also important in terms of the impact on health, certain people can consume only specific fishes, because of allergic sensitivity. The most common determination of fish species is based on morphological traits. This approach faces more and more complications as the level of processing fish flesh into products of food industry and/or complex dishes in gastronomy makes morphological markers less available. Methods based on the polymerase chain reaction (PCR) are the most spread for control purposes, due to the high level of sensitivity and specificity. The presented analysis is performed as an amplification of nuclear gene encoding important protein of fish muscles parvalbumin, also known as a major fish allergen. Conventional PCR method for the differentiation of the following fish species were developed: black seabream (Sponyliosoma cantharus), Atlantic mackerel (Scomber scombrus), iridescent shark (Pangasianodon hypophthalmus) and angler fish (Lophius piscatorius). Specificity was verified on panel of 19 different fish species. This method can be employed as a routine tool for species origin determination irrespectively of morphological traits.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40103 - Fishery

Projekt

<a href="/cs/project/QK1910231" target="_blank" >QK1910231: Nové přístupy k průkazu falšování rybího masa pomocí genomové DNA</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů