Conference poster: Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F21%3AN0000008" target="_blank" >RIV/00027022:_____/21:N0000008 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Conference poster: Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering

Popis výsledku v původním jazyce

Soft foams with micro-macro porous hierarchical structure have been prepared by freeze-drying of solutions of polylactide (PLA, Ingeo 4043D, Mn 160.000 g.mol-1) and polycaprolactone (PCL, Aldrich, Mn 80.000 g.mol-1) mixtures in 1,4-dioxane with or without addition of sucrose porogens. The foams prepared by freeze-drying had open-porous aerogel characteristics. Pore sizes decreased with increasing the concentration of the polymers in solution in the range from several micrometers to several hundreds of micrometers. The hierarchical structure with bimodal micro-macro pore size distribution were obtained after addition of sucrose porogens with defined crystal size distributions to the solutions before freeze-drying and leaching the sucrose crystals from the freeze-dried foams with demineralised water. Polyethyleneimine was chemically conjugated to the alkali-treated foams followed by conjugation of citric acid using carbodiimide (EDC-NHS) chemistry. Finally, they were mineralised by immersing and incubating in a simulated body fluid with ionic concentration similar to that of human blood plasma, to obtain tissue engineering scaffolds. To verify their biocompatibility, the scaffolds were incubated with adipose-derived stem cells (ASC). Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test.

Název v anglickém jazyce

Conference poster: Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering

Popis výsledku anglicky

Soft foams with micro-macro porous hierarchical structure have been prepared by freeze-drying of solutions of polylactide (PLA, Ingeo 4043D, Mn 160.000 g.mol-1) and polycaprolactone (PCL, Aldrich, Mn 80.000 g.mol-1) mixtures in 1,4-dioxane with or without addition of sucrose porogens. The foams prepared by freeze-drying had open-porous aerogel characteristics. Pore sizes decreased with increasing the concentration of the polymers in solution in the range from several micrometers to several hundreds of micrometers. The hierarchical structure with bimodal micro-macro pore size distribution were obtained after addition of sucrose porogens with defined crystal size distributions to the solutions before freeze-drying and leaching the sucrose crystals from the freeze-dried foams with demineralised water. Polyethyleneimine was chemically conjugated to the alkali-treated foams followed by conjugation of citric acid using carbodiimide (EDC-NHS) chemistry. Finally, they were mineralised by immersing and incubating in a simulated body fluid with ionic concentration similar to that of human blood plasma, to obtain tissue engineering scaffolds. To verify their biocompatibility, the scaffolds were incubated with adipose-derived stem cells (ASC). Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

<a href="/cs/project/NU20-08-00208" target="_blank" >NU20-08-00208: Nové vaskularizované konstrukty na bázi kmenových buněk pro inženýrství měkkých a tvrdých tkání</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů