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Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00567467" target="_blank" >RIV/67985823:_____/22:00567467 - isvavai.cz</a>

  • Nalezeny alternativní kódy

    RIV/00027022:_____/21:N0000009 RIV/67985823:_____/21:00567467

  • Výsledek na webu

    <a href="https://www.confer.cz/nanocon/2021/read/4346-mineralised-polylactide-and-polycaprolactone-soft-foams-with-hierarchical-micro-macro-porous-structure-for-tissue-engineering.pdf" target="_blank" >https://www.confer.cz/nanocon/2021/read/4346-mineralised-polylactide-and-polycaprolactone-soft-foams-with-hierarchical-micro-macro-porous-structure-for-tissue-engineering.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.37904/nanocon.2021.4346" target="_blank" >10.37904/nanocon.2021.4346</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering

  • Popis výsledku v původním jazyce

    The purpose of the study was development of soft foams from resorbable polymers with unique micro-macro porous interconnected hierarchical structure specially designed as scaffold for engineering of soft tissues. The foams have been prepared by freeze-drying of solutions of polylactide (PLA) and polycaprolactone (PCL) in 1,4-dioxane. The foams prepared by freeze-drying had interconnected porous aerogel characteristics. The hierarchical structure with bimodal micro-macro pore size distribution were obtained after addition of sucrose or NaCl porogens with defined crystal size distributions to the solutions before freeze-drying and leaching the porogen crystals from the freeze-dried foams with demineralised water. Polyethyleneimine was chemically conjugated to the alkali-treated foams followed by conjugation of citric acid using carbodiimide chemistry. Finally, they were mineralised by immersing and incubating in a simulated body fluid with ionic concentration similar to that of human blood plasma, to obtain tissue engineering scaffolds. To verify their biocompatibility, the scaffolds were seeded with adipose-derived stem cells (ASC) and sarcoma osteogenic-2 (SaOs-2) human osteoblast-like cells. Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test. Biocompatibility and usability of the scaffolds was successfully verified by incubation with adipose-derived stem cells and SaOs-2 human osteosarcoma cell line. Mineralised scaffolds are more suitable growth supports for both the cell types than unmineralized collagen scaffolds. The scaffolds have been specially designed for engineering of soft tissues, but they can be used in other categories of tissue engineering, too.

  • Název v anglickém jazyce

    Mineralised polylactide and polycaprolactone soft foams with hierarchical micro-macro porous structure for tissue engineering

  • Popis výsledku anglicky

    The purpose of the study was development of soft foams from resorbable polymers with unique micro-macro porous interconnected hierarchical structure specially designed as scaffold for engineering of soft tissues. The foams have been prepared by freeze-drying of solutions of polylactide (PLA) and polycaprolactone (PCL) in 1,4-dioxane. The foams prepared by freeze-drying had interconnected porous aerogel characteristics. The hierarchical structure with bimodal micro-macro pore size distribution were obtained after addition of sucrose or NaCl porogens with defined crystal size distributions to the solutions before freeze-drying and leaching the porogen crystals from the freeze-dried foams with demineralised water. Polyethyleneimine was chemically conjugated to the alkali-treated foams followed by conjugation of citric acid using carbodiimide chemistry. Finally, they were mineralised by immersing and incubating in a simulated body fluid with ionic concentration similar to that of human blood plasma, to obtain tissue engineering scaffolds. To verify their biocompatibility, the scaffolds were seeded with adipose-derived stem cells (ASC) and sarcoma osteogenic-2 (SaOs-2) human osteoblast-like cells. Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test. Biocompatibility and usability of the scaffolds was successfully verified by incubation with adipose-derived stem cells and SaOs-2 human osteosarcoma cell line. Mineralised scaffolds are more suitable growth supports for both the cell types than unmineralized collagen scaffolds. The scaffolds have been specially designed for engineering of soft tissues, but they can be used in other categories of tissue engineering, too.

Klasifikace

  • Druh

    D - Stať ve sborníku

  • CEP obor

  • OECD FORD obor

    30404 - Biomaterials (as related to medical implants, devices, sensors)

Návaznosti výsledku

  • Projekt

    <a href="/cs/project/NU20-08-00208" target="_blank" >NU20-08-00208: Nové vaskularizované konstrukty na bázi kmenových buněk pro inženýrství měkkých a tvrdých tkání</a><br>

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Ostatní

  • Rok uplatnění

    2022

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název statě ve sborníku

    NANOCON 2021 - Conference proceedings

  • ISBN

    978-80-88365-00-6

  • ISSN

    2694-930X

  • e-ISSN

  • Počet stran výsledku

    6

  • Strana od-do

    298-303

  • Název nakladatele

    Tanger Ltd.

  • Místo vydání

    Ostrava

  • Místo konání akce

    Brno

  • Datum konání akce

    20. 10. 2021

  • Typ akce podle státní příslušnosti

    WRD - Celosvětová akce

  • Kód UT WoS článku