Soft and hard polymeric foams for tissue engineering

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F23%3AN0000086" target="_blank" >RIV/00027022:_____/23:N0000086 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Soft and hard polymeric foams for tissue engineering

Popis výsledku v původním jazyce

In this work, two different types of foam based biodegradable scaffolds for bone and soft tissue engineering with a fully interconnected porous network have been prepared: 1) soft polymeric foams with hierarchical micro-macro porous structure reinforced by addition of nanoparticular inorganic reinforcement fillers; 2) 3D printed polymeric scaffolds. Soft foams with micro-macro porous hierarchical structure have been prepared by freeze-drying of solutions of polylactide (PLA), polycaprolactone (PCL) or their mixtures in dioxane. The foams prepared by freeze-drying had interconnected porous aerogel characteristics. The hierarchical structure with bimodal micro-macro pore size distribution was obtained after addition of NaCl porogen to the solutions before freeze-drying and leaching the crystals from the freeze-dried foams with demineralised water. Additive manufacturing techniques enable to fabricate polymeric scaffolds with tailored architecture, to control its functionality and to optimize the parameters of the scaffolds and create structures with desired characteristics like controllable biodegradability and mechanical stability along with a precise pore size and high permeability for purposes of osteoinduction, osseointegration and angiogenesis. Ten samples of the hard foams with different inner geometries were printed from natural PLA. To verify their biocompatibility, the scaffolds were seeded with human osteoblast-like SAOS-2 cells. Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test.

Název v anglickém jazyce

Soft and hard polymeric foams for tissue engineering

Popis výsledku anglicky

In this work, two different types of foam based biodegradable scaffolds for bone and soft tissue engineering with a fully interconnected porous network have been prepared: 1) soft polymeric foams with hierarchical micro-macro porous structure reinforced by addition of nanoparticular inorganic reinforcement fillers; 2) 3D printed polymeric scaffolds. Soft foams with micro-macro porous hierarchical structure have been prepared by freeze-drying of solutions of polylactide (PLA), polycaprolactone (PCL) or their mixtures in dioxane. The foams prepared by freeze-drying had interconnected porous aerogel characteristics. The hierarchical structure with bimodal micro-macro pore size distribution was obtained after addition of NaCl porogen to the solutions before freeze-drying and leaching the crystals from the freeze-dried foams with demineralised water. Additive manufacturing techniques enable to fabricate polymeric scaffolds with tailored architecture, to control its functionality and to optimize the parameters of the scaffolds and create structures with desired characteristics like controllable biodegradability and mechanical stability along with a precise pore size and high permeability for purposes of osteoinduction, osseointegration and angiogenesis. Ten samples of the hard foams with different inner geometries were printed from natural PLA. To verify their biocompatibility, the scaffolds were seeded with human osteoblast-like SAOS-2 cells. Morphology of the cells attached to the scaffolds was evaluated and their viability was verified by a metabolic test.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30404 - Biomaterials (as related to medical implants, devices, sensors)

Projekt

<a href="/cs/project/NU20-08-00208" target="_blank" >NU20-08-00208: Nové vaskularizované konstrukty na bázi kmenových buněk pro inženýrství měkkých a tvrdých tkání</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů