Chitin quantitation (as glucosamine) in food raw materials by spectrophotometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027022%3A_____%2F24%3AN0000013" target="_blank" >RIV/00027022:_____/24:N0000013 - isvavai.cz</a>

Výsledek na webu

<a href="https://methods-x.com/article/S2215-0161(24)00123-7/fulltext" target="_blank" >https://methods-x.com/article/S2215-0161(24)00123-7/fulltext</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mex.2024.102669" target="_blank" >10.1016/j.mex.2024.102669</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Chitin quantitation (as glucosamine) in food raw materials by spectrophotometry

Popis výsledku v původním jazyce

Chitin is a water insoluble nitrogen-containing polysaccharide made from N-acetyl-D-glucosamine¨containing β-(1→4)-linkages. In food, chitin is considered as a source of fiber with prebiotic properties to gut microflora. Chitin content varies widely in nature from 1% (yeasts) up to 64% (butterfly cuticles) and is mostly found in filamentous or mushroom forming fungi, insects and crustaceans. This spectrophotometric method is suitable for chitin quantitation (reported as glucosamine) in food raw materials like insects (mealworm larvae, crickets), shrimps, mushrooms and fungi in a research (non-routine) laboratory. To remove interferences, the sample is defatted (Soxhlet) prior to acid hydrolysis in 6 M HCl. The color complex is developed after the addition of Katano’s reagent (a mix of 0.05 mol/L sodium metasilicate, 0.6 mol/L sodium molybdate, 30% dimethyl sulfoxide and 1.42 mol/L acetic acid) at 70 °C for 30 min and measured at 750 nm against blank. A five-point linear calibration (5–100 μg/mL) is used. Limit of detection is 3 μg GLCN/mL. The correlation (R2) with an HPLC method for chitin analysis is at least 0.93. • a reliable alternative to an HPLC method • does not require expensive equipment • deproteination by alkali is not necessary for most matrices - saves about 30% of time

Název v anglickém jazyce

Chitin quantitation (as glucosamine) in food raw materials by spectrophotometry

Popis výsledku anglicky

Chitin is a water insoluble nitrogen-containing polysaccharide made from N-acetyl-D-glucosamine¨containing β-(1→4)-linkages. In food, chitin is considered as a source of fiber with prebiotic properties to gut microflora. Chitin content varies widely in nature from 1% (yeasts) up to 64% (butterfly cuticles) and is mostly found in filamentous or mushroom forming fungi, insects and crustaceans. This spectrophotometric method is suitable for chitin quantitation (reported as glucosamine) in food raw materials like insects (mealworm larvae, crickets), shrimps, mushrooms and fungi in a research (non-routine) laboratory. To remove interferences, the sample is defatted (Soxhlet) prior to acid hydrolysis in 6 M HCl. The color complex is developed after the addition of Katano’s reagent (a mix of 0.05 mol/L sodium metasilicate, 0.6 mol/L sodium molybdate, 30% dimethyl sulfoxide and 1.42 mol/L acetic acid) at 70 °C for 30 min and measured at 750 nm against blank. A five-point linear calibration (5–100 μg/mL) is used. Limit of detection is 3 μg GLCN/mL. The correlation (R2) with an HPLC method for chitin analysis is at least 0.93. • a reliable alternative to an HPLC method • does not require expensive equipment • deproteination by alkali is not necessary for most matrices - saves about 30% of time

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MethodsX

ISSN

22150161

e-ISSN

—

Svazek periodika

12

Číslo periodika v rámci svazku

102669

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

1-7

Kód UT WoS článku

001222725900001

EID výsledku v databázi Scopus

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