Electrophoretic determination of chitin in insects

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60460709%3A41210%2F23%3A95082" target="_blank" >RIV/60460709:41210/23:95082 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/23:43928048

Výsledek na webu

<a href="https://doi.org/10.1016/j.chroma.2023.463952" target="_blank" >https://doi.org/10.1016/j.chroma.2023.463952</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2023.463952" target="_blank" >10.1016/j.chroma.2023.463952</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrophoretic determination of chitin in insects

Popis výsledku v původním jazyce

An electrophoretic method (on-line coupled capillary isotachophoresis with capillary zone electrophoresis with conductometric detection (cITP-CZE-COND)) for the determination of chitin in insects based on the analysis of glucosamine after acidic hydrolysis of the sample is described. Chitin is deacetylated and hydrolyzed to glucosamine by acidic hydrolysis (6 M sulfuric acid, 110 degrees C, 6 h). Under optimized electrophoresis conditions, glucosamine (GlcN) is separated from other sample components in cationic mode and detected with a conductometer within 15 min. The performance method characteristics of the GlcN assay, i.e. , linearity (0.2-20 mu mol), accuracy (103 +/- 5%), repeatability (1.9%), reproducibility (3.4%), limits of detection (0.06 mu mol/L) and quantification (0.2 mu mol/L), were evaluated. On a series of 28 insect samples, it was proven that cITP-CZE-COND provides results of chitin content in insects comparable to the literature data. The important features of the developed cITP-CZE-COND method are easy sample treatment, high sensitivity and selectivity, and low running costs. It is clear from the above that the cITP-CZE-COND method is suitable for analysis of insect samples for chitin content. (c) 2023 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Electrophoretic determination of chitin in insects

Popis výsledku anglicky

An electrophoretic method (on-line coupled capillary isotachophoresis with capillary zone electrophoresis with conductometric detection (cITP-CZE-COND)) for the determination of chitin in insects based on the analysis of glucosamine after acidic hydrolysis of the sample is described. Chitin is deacetylated and hydrolyzed to glucosamine by acidic hydrolysis (6 M sulfuric acid, 110 degrees C, 6 h). Under optimized electrophoresis conditions, glucosamine (GlcN) is separated from other sample components in cationic mode and detected with a conductometer within 15 min. The performance method characteristics of the GlcN assay, i.e. , linearity (0.2-20 mu mol), accuracy (103 +/- 5%), repeatability (1.9%), reproducibility (3.4%), limits of detection (0.06 mu mol/L) and quantification (0.2 mu mol/L), were evaluated. On a series of 28 insect samples, it was proven that cITP-CZE-COND provides results of chitin content in insects comparable to the literature data. The important features of the developed cITP-CZE-COND method are easy sample treatment, high sensitivity and selectivity, and low running costs. It is clear from the above that the cITP-CZE-COND method is suitable for analysis of insect samples for chitin content. (c) 2023 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

0021-9673

Svazek periodika

1695

Číslo periodika v rámci svazku

April 26 2023

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

1-10

Kód UT WoS článku

000967379900001

EID výsledku v databázi Scopus

2-s2.0-85151243548