Imunohistochemická detekce centrální nervové tkáně v masných výrobcích

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F05%3A06000046" target="_blank" >RIV/00027162:_____/05:06000046 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immunohistochemical detection of central nervous tissue in meat products

Popis výsledku v původním jazyce

The aim of the study was to test applicability of commercially-available antibodies, intended for human tissues, for immunohistochemical detection of central nervous system (CNS) tissue from farm animals in meat products. Sensitivity and specificity ofimmunoreactions of seven antibodies for the detection of neurofilaments, betaIII-tubulin, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were tested. Specific immunoreactions were shown only by monoclonal anti-GFAP (ICNBiomedicals) and polyclonal anti-MBP (DakoCytomation) antibodies; the other antibodies demonstrated cross-reactivity with other tissues. Neither of the antibodies is species specific (cattle, pig, sheep, goat, hen). In heat treated samples of CNS tissueand in sausagescontaining 0, 1, 5 and 10% of cattle brain tissue, no marked weakening of immunoreaction was recorded only for MBP detection.

Název v anglickém jazyce

Immunohistochemical detection of central nervous tissue in meat products

Popis výsledku anglicky

The aim of the study was to test applicability of commercially-available antibodies, intended for human tissues, for immunohistochemical detection of central nervous system (CNS) tissue from farm animals in meat products. Sensitivity and specificity ofimmunoreactions of seven antibodies for the detection of neurofilaments, betaIII-tubulin, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP) were tested. Specific immunoreactions were shown only by monoclonal anti-GFAP (ICNBiomedicals) and polyclonal anti-MBP (DakoCytomation) antibodies; the other antibodies demonstrated cross-reactivity with other tissues. Neither of the antibodies is species specific (cattle, pig, sheep, goat, hen). In heat treated samples of CNS tissueand in sausagescontaining 0, 1, 5 and 10% of cattle brain tissue, no marked weakening of immunoreaction was recorded only for MBP detection.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

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Svazek periodika

74

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

449-454

Kód UT WoS článku

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EID výsledku v databázi Scopus

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