Identifikace předchůdců multirezistentních kmenů Salmonella enterica serovar Typhimurium fágového typu DT104

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F07%3A%230000179" target="_blank" >RIV/00027162:_____/07:#0000179 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/07:00050770

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of putative ancestors of the multidrug-resistant Salmonella enterica serovar typhimurium DT104 clone harboring the Salmonella genomic island 1

Popis výsledku v původním jazyce

Origin of multidrug-resistant S. typhimurium harboring the SGI1 is unknown. In this study, we performed microarray genomotyping of four multidrug-resistant SGI1 positive strains and found that unlike the S. typhimurium LT2 strain, the multidrug-resistantstrains lacked genes STM0517-0529. We extended this observation by PCR screening of additional 120 S. typhimurium field strains and found that this locus was absent in all SGI1 positive and also in 24% of SGI1 negative strains which were proposed to bethe original recipients of SGI1. To prove this hypothesis, we compared the STM0517-0529 negative strains (with or without the SGI1) by PFGE and PCR prophage typing and found that 8 out of 11 of the SGI1 negative strains and 17 out of 22 SGI1 positive strains were of identical PFGE pattern and PCR prophage pattern. We therefore proposed that a lineage of the S. typhimurium DT104 sensitive strain first lost STM0517-0529 genes and then acquired SGI1.

Název v anglickém jazyce

Identification of putative ancestors of the multidrug-resistant Salmonella enterica serovar typhimurium DT104 clone harboring the Salmonella genomic island 1

Popis výsledku anglicky

Origin of multidrug-resistant S. typhimurium harboring the SGI1 is unknown. In this study, we performed microarray genomotyping of four multidrug-resistant SGI1 positive strains and found that unlike the S. typhimurium LT2 strain, the multidrug-resistantstrains lacked genes STM0517-0529. We extended this observation by PCR screening of additional 120 S. typhimurium field strains and found that this locus was absent in all SGI1 positive and also in 24% of SGI1 negative strains which were proposed to bethe original recipients of SGI1. To prove this hypothesis, we compared the STM0517-0529 negative strains (with or without the SGI1) by PFGE and PCR prophage typing and found that 8 out of 11 of the SGI1 negative strains and 17 out of 22 SGI1 positive strains were of identical PFGE pattern and PCR prophage pattern. We therefore proposed that a lineage of the S. typhimurium DT104 sensitive strain first lost STM0517-0529 genes and then acquired SGI1.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of Microbiology

ISSN

0302-8933

e-ISSN

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Svazek periodika

187

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

415-424

Kód UT WoS článku

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EID výsledku v databázi Scopus

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