Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000496" target="_blank" >RIV/00027162:_____/09:#0000496 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea

Popis výsledku v původním jazyce

The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms. Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as porcine enterovirus serotype 1 based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection ofthe genus Teschovirus (PTV) and the Porcine teschovirus serotype 1 (PTV-1). The fragment characteristic for PTV was detected in all samples. The fragment characteristic for PTV-1 was detected with the exception of three strains. Used RT-PCR method was subsequently applied to the detection of PTV in pig fecal samples. Ten of 22 faecal samples were found to be PTV positive, however none of 22 samples generated amplicon specific for the PTV-1.

Název v anglickém jazyce

Genotyping of porcine teschoviruses isolated from 1960 to 1980 in the former Czechoslovakia and new Porcine teschovirus isolates obtained from piglets with diarrhoea

Popis výsledku anglicky

The aim of the study was retyping and reclassification of the 27 strains of porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms. Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as porcine enterovirus serotype 1 based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection ofthe genus Teschovirus (PTV) and the Porcine teschovirus serotype 1 (PTV-1). The fragment characteristic for PTV was detected in all samples. The fragment characteristic for PTV-1 was detected with the exception of three strains. Used RT-PCR method was subsequently applied to the detection of PTV in pig fecal samples. Ten of 22 faecal samples were found to be PTV positive, however none of 22 samples generated amplicon specific for the PTV-1.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

54

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

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Kód UT WoS článku

000272392800002

EID výsledku v databázi Scopus

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