Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F09%3A%230000566" target="_blank" >RIV/00027162:_____/09:#0000566 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro

Popis výsledku v původním jazyce

The aim of this study was to assess functional status of spermatozoa after cryopreservation, separation and capacitation to characterize the influence of these procedures on spermatozoa functional parameters. Boar semen were frozen-thawed, motile spermatozoa were separated on Percoll-gradient and capacitated by 1mM caffeine. Motility, viability and chromatine integrity of spermatozoa were evaluated by phase contrast, Annexin V-FICT apoptosis kit and SCSA assay respectively. The high cryotolerant- (HCT)and the low cryotolerant- (LCT) boars were classified in previous experiments. There were no differences in motility and viability spermatozoa between both boar groups before freezing but after thawing, the significantly higher motility and viability were found for HCT- compared with those for LCT-boars. Separation was more effective in the LCT- than in HCT- boars.. In both groups, DNA of spermatozoa was highly stable during cryopreservation, separation and capacitation.

Název v anglickém jazyce

Changes in functional parameters of cryopreserved boar spermatozoa capacitated in vitro

Popis výsledku anglicky

The aim of this study was to assess functional status of spermatozoa after cryopreservation, separation and capacitation to characterize the influence of these procedures on spermatozoa functional parameters. Boar semen were frozen-thawed, motile spermatozoa were separated on Percoll-gradient and capacitated by 1mM caffeine. Motility, viability and chromatine integrity of spermatozoa were evaluated by phase contrast, Annexin V-FICT apoptosis kit and SCSA assay respectively. The high cryotolerant- (HCT)and the low cryotolerant- (LCT) boars were classified in previous experiments. There were no differences in motility and viability spermatozoa between both boar groups before freezing but after thawing, the significantly higher motility and viability were found for HCT- compared with those for LCT-boars. Separation was more effective in the LCT- than in HCT- boars.. In both groups, DNA of spermatozoa was highly stable during cryopreservation, separation and capacitation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GI - Šlechtění a plemenářství hospodářských zvířat

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Pig Breeding

ISSN

1802-7547

e-ISSN

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Svazek periodika

3

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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