Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F10%3A%230000619" target="_blank" >RIV/00027162:_____/10:#0000619 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization

Popis výsledku v původním jazyce

The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction (AR) development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1 mM and 2 mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability,chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. It can be concluded that there is a potential relationship between the acrosome reaction progress in frozen-thawed boar spermatozoa and the efficiency of fertilization of porcine oocytes. A faster AR induced in spermatozoa by 1 mM caffeine resulted in a higher monospermy rate and total efficiency of fertilization. It is important to test sires before their semen is used for in vitro embryo production.

Název v anglickém jazyce

Effect of acrosome reaction progress in frozen-thawed boar spermatozoa on the efficiency of in vitro oocyte fertilization

Popis výsledku anglicky

The purpose of the study was to evaluate the changes in functional status of boar spermatozoa separated from frozen-thawed semen and capacitated in vitro by caffeine. The effect of acrosome reaction (AR) development in spermatozoa on the efficiency of oocyte fertilization has been studied in boars A B and C. Motile spermatozoa were separated by Percoll gradient, untreated (control) or treated with both 1 mM and 2 mM caffeine, and capacitated or co-cultured with matured oocytes. The motility, viability,chromatin and acrosome integrity, and fertilizing ability of spermatozoa were assessed. It can be concluded that there is a potential relationship between the acrosome reaction progress in frozen-thawed boar spermatozoa and the efficiency of fertilization of porcine oocytes. A faster AR induced in spermatozoa by 1 mM caffeine resulted in a higher monospermy rate and total efficiency of fertilization. It is important to test sires before their semen is used for in vitro embryo production.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární Medicína

ISSN

0375-8427

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000282708500003

EID výsledku v databázi Scopus

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