Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000743" target="_blank" >RIV/00027162:_____/11:#0000743 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR

Popis výsledku v původním jazyce

This study focused on the development of a DNA isolation procedure for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces by IS900 and F57 quantitative real time PCR (qPCR) and their comparison with culture. The recovery of MAPDNA ranged from 29.1 to 102.4% of the input amount of MAP with median 37.9%. The limit of detection was determined to be 1.03 x 10(4) for F57 qPCR and 6.87 x 10(2) MAP cells per gram of faeces for IS900 qPCR, respectively. The developed technique for DNAisolation was coupled with 15900 qPCR and compared to traditional MAP culture using a cohort of 1906 faecal samples examined from 12 dairy cattle farms in our laboratory. From those 1906 original faecal samples. 875 were positive by IS900 qPCR and 169 by culture. This data facilitated development of a predictive model capable of estimating the probability of being culture positive by estimating the absolute number of MAP per gram of faeces as determined IS900 qPCR without performing the

Název v anglickém jazyce

Development of a predictive model for detection of Mycobacterium avium subsp paratuberculosis in faeces by quantitative real time PCR

Popis výsledku anglicky

This study focused on the development of a DNA isolation procedure for the detection of Mycobacterium avium subsp. paratuberculosis (MAP) in faeces by IS900 and F57 quantitative real time PCR (qPCR) and their comparison with culture. The recovery of MAPDNA ranged from 29.1 to 102.4% of the input amount of MAP with median 37.9%. The limit of detection was determined to be 1.03 x 10(4) for F57 qPCR and 6.87 x 10(2) MAP cells per gram of faeces for IS900 qPCR, respectively. The developed technique for DNAisolation was coupled with 15900 qPCR and compared to traditional MAP culture using a cohort of 1906 faecal samples examined from 12 dairy cattle farms in our laboratory. From those 1906 original faecal samples. 875 were positive by IS900 qPCR and 169 by culture. This data facilitated development of a predictive model capable of estimating the probability of being culture positive by estimating the absolute number of MAP per gram of faeces as determined IS900 qPCR without performing the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinary Microbiology

ISSN

0378-1135

e-ISSN

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Svazek periodika

149

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

133-138

Kód UT WoS článku

000289031600016

EID výsledku v databázi Scopus

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