Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F11%3A%230000754" target="_blank" >RIV/00027162:_____/11:#0000754 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms

Popis výsledku v původním jazyce

Staphylococcus epidermidis may form biofilms in milk processing plants and secondarily contaminate milk products. In this study, the ability of S. epidermidis to form a biofilm in milk and the influence of glucose and NaCl on biofilm formation were analysed. Biofilm production on stainless steel was investigated at 30 degrees C in pasteurized milk and in milk containing glucose, NaCl or both glucose and NaCl. Biofilm formation was evaluated by monitoring of colony-forming units (cfu) on stainless chipsand by real-time quantitative PCR analysis of the icaA and sigma(B) transcripts. An increase in cfu and icaA transcription was observed after 20 h incubation, especially in milk containing glucose and NaCl. NaCl alone was shown to present a greater riskof biofilm development in milk than glucose alone, but the NaCl/glucose combination represented the highest risk. The sigma(B) factor did not seem to be involved in biofilm formation under the conditions of this study.

Název v anglickém jazyce

Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms Biofilm formation on stainless steel by Staphylococcus epidermidis in milk and influence of glucose and sodium chloride on the development of ica-mediated biofilms

Popis výsledku anglicky

Staphylococcus epidermidis may form biofilms in milk processing plants and secondarily contaminate milk products. In this study, the ability of S. epidermidis to form a biofilm in milk and the influence of glucose and NaCl on biofilm formation were analysed. Biofilm production on stainless steel was investigated at 30 degrees C in pasteurized milk and in milk containing glucose, NaCl or both glucose and NaCl. Biofilm formation was evaluated by monitoring of colony-forming units (cfu) on stainless chipsand by real-time quantitative PCR analysis of the icaA and sigma(B) transcripts. An increase in cfu and icaA transcription was observed after 20 h incubation, especially in milk containing glucose and NaCl. NaCl alone was shown to present a greater riskof biofilm development in milk than glucose alone, but the NaCl/glucose combination represented the highest risk. The sigma(B) factor did not seem to be involved in biofilm formation under the conditions of this study.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Dairy Journal

ISSN

0958-6946

e-ISSN

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Svazek periodika

21

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

179-184

Kód UT WoS článku

000286189200008

EID výsledku v databázi Scopus

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