ClpP deletion causes attenuation of Salmonella Typhimurium virulence through mis-regulation of RpoS and indirect control of CsrA and the SPI genes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F13%3A%230001034" target="_blank" >RIV/00027162:_____/13:#0001034 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1099/mic.0.065797-0" target="_blank" >http://dx.doi.org/10.1099/mic.0.065797-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1099/mic.0.065797-0" target="_blank" >10.1099/mic.0.065797-0</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
ClpP deletion causes attenuation of Salmonella Typhimurium virulence through mis-regulation of RpoS and indirect control of CsrA and the SPI genes
Popis výsledku v původním jazyce
Salmonella enterica serovar Typhimurium requires the type III secretion system encoded by Salmonella pathogenicity island 1 (SPI1) and controlled by the master regulator, HilA, to penetrate the intestinal epithelium. Numerous regulators affect virulencethrough influence on this system, including the proteolytic component ClpP, the stationary phase regulator RpoS and the carbon-storage regulator CsrA. However, the mechanism behind the ClpP regulation is not fully understood. To elucidate this we examined differentially expressed genes in a Delta clpP mutant compared with WT using global transcriptomic analysis. SPI1 and SPI4 virulence genes were significantly downregulated in the Delta clpP mutant, whereas several RpoS-dependent genes and the fliC geneencoding flagellin were upregulated. While the Delta clpP mutant was attenuated in cell invasion, this attenuation was not present in a Delta clpP/rpoS:: amp double mutant, suggesting the repression of invasion was directed through RpoS.
Název v anglickém jazyce
ClpP deletion causes attenuation of Salmonella Typhimurium virulence through mis-regulation of RpoS and indirect control of CsrA and the SPI genes
Popis výsledku anglicky
Salmonella enterica serovar Typhimurium requires the type III secretion system encoded by Salmonella pathogenicity island 1 (SPI1) and controlled by the master regulator, HilA, to penetrate the intestinal epithelium. Numerous regulators affect virulencethrough influence on this system, including the proteolytic component ClpP, the stationary phase regulator RpoS and the carbon-storage regulator CsrA. However, the mechanism behind the ClpP regulation is not fully understood. To elucidate this we examined differentially expressed genes in a Delta clpP mutant compared with WT using global transcriptomic analysis. SPI1 and SPI4 virulence genes were significantly downregulated in the Delta clpP mutant, whereas several RpoS-dependent genes and the fliC geneencoding flagellin were upregulated. While the Delta clpP mutant was attenuated in cell invasion, this attenuation was not present in a Delta clpP/rpoS:: amp double mutant, suggesting the repression of invasion was directed through RpoS.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíne</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
MICROBIOLOGY-SGM
ISSN
1350-0872
e-ISSN
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Svazek periodika
159
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
13
Strana od-do
1497-1509
Kód UT WoS článku
000322855700025
EID výsledku v databázi Scopus
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