Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001093" target="_blank" >RIV/00027162:_____/14:#0001093 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4315/0362-028X.JFP-13-036" target="_blank" >http://dx.doi.org/10.4315/0362-028X.JFP-13-036</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4315/0362-028X.JFP-13-036" target="_blank" >10.4315/0362-028X.JFP-13-036</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

Popis výsledku v původním jazyce

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB) like lesions from 17 pig carcasses, as well as samples of head and mesentericlymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (10(2) to 10(3) cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantifica

Název v anglickém jazyce

Mycobacterium avium subsp avium in Lymph Nodes and Diaphragms of Pigs from One Infected Herd in the Czech Republic

Popis výsledku anglicky

This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB) like lesions from 17 pig carcasses, as well as samples of head and mesentericlymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (10(2) to 10(3) cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantifica

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Protection

ISSN

0362-028X

e-ISSN

—

Svazek periodika

77

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

141-144

Kód UT WoS článku

000329593300020

EID výsledku v databázi Scopus

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