Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001150" target="_blank" >RIV/00027162:_____/14:#0001150 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/14:#0001330

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.talanta.2014.02.007" target="_blank" >http://dx.doi.org/10.1016/j.talanta.2014.02.007</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2014.02.007" target="_blank" >10.1016/j.talanta.2014.02.007</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods

Popis výsledku v původním jazyce

The analytical performance and evaluation of a kit-based ELISA for the determination of acrylamide in fried potato and corn chip samples are described. The sample homogenate is subjected to clean-up using SPE, followed by analyte derivatization and ELISAdetection. Accuracy, precision and linearity of the ELISA procedure have been validated using spiked samples. Analytical recovery ranged from 91.8% to 96.0% with coefficients of variation below 15%. Good linearity over a wide range of dilution and minimal assay drift was observed within a microtiter plate. IC50 value of the calibration curve was 110 ng/mL, with the limit of detection about 5 ng/mL and dynamic range from 10 to 1000 ng/mL. The high specificity of the ELISA was demonstrated by cross-reactivity study using 11 potential cross-reactants. A good correlation between the results obtained from the ELISA and GC-MS within the concentration range 120-1500 mu g/kg was found in the chip samples (r=0.992, n=120). The data demonstrate

Název v anglickém jazyce

Analytical evaluation of a high-throughput enzyme-linked immunosorbent assay for acrylamide determination in fried foods

Popis výsledku anglicky

The analytical performance and evaluation of a kit-based ELISA for the determination of acrylamide in fried potato and corn chip samples are described. The sample homogenate is subjected to clean-up using SPE, followed by analyte derivatization and ELISAdetection. Accuracy, precision and linearity of the ELISA procedure have been validated using spiked samples. Analytical recovery ranged from 91.8% to 96.0% with coefficients of variation below 15%. Good linearity over a wide range of dilution and minimal assay drift was observed within a microtiter plate. IC50 value of the calibration curve was 110 ng/mL, with the limit of detection about 5 ng/mL and dynamic range from 10 to 1000 ng/mL. The high specificity of the ELISA was demonstrated by cross-reactivity study using 11 potential cross-reactants. A good correlation between the results obtained from the ELISA and GC-MS within the concentration range 120-1500 mu g/kg was found in the chip samples (r=0.992, n=120). The data demonstrate

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

—

Projekt

<a href="/cs/project/ED0006%2F01%2F01" target="_blank" >ED0006/01/01: Centrum pro aplikovanou mikrobiologii a imunologii ve veterinární medicíne</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

—

Svazek periodika

123

Číslo periodika v rámci svazku

JUN

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

5

Strana od-do

146-150

Kód UT WoS článku

000335616300021

EID výsledku v databázi Scopus

—