Quantitative immunohistochemical method for detection of wheat protein in model sausage
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F14%3A%230001352" target="_blank" >RIV/00027162:_____/14:#0001352 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/62157124:16270/15:43874681
Výsledek na webu
<a href="http://actavet.vfu.cz/media/pdf/avb_2014083100071.pdf" target="_blank" >http://actavet.vfu.cz/media/pdf/avb_2014083100071.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2754/avb201483S10S71" target="_blank" >10.2754/avb201483S10S71</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Quantitative immunohistochemical method for detection of wheat protein in model sausage
Popis výsledku v původním jazyce
Since gluten can induce coeliac symptoms in hypersensitive consumers with coeliac disease, it is necessary to label foodstuffs containing it. In order to label foodstuffs, it is essential to find reliable methods to accurately determine the amount of wheat protein in food. The objective of this study was to compare the quantitative detection of wheat protein in model sausages by ELISA and immunohistochemical methods. Immunohistochemistry was combined with stereology to achieve quantitative results. High correlation between addition of wheat protein and compared methods was confirmed. For ELISA method the determined values were r = 0.98, P < 0.01; for stereologythe determined values were r = 0.94, P < 0.01. Although ELISA is an accredited method, it was not reliable, unlike immunohistochemical methods (stereology SD = 3.1).
Název v anglickém jazyce
Quantitative immunohistochemical method for detection of wheat protein in model sausage
Popis výsledku anglicky
Since gluten can induce coeliac symptoms in hypersensitive consumers with coeliac disease, it is necessary to label foodstuffs containing it. In order to label foodstuffs, it is essential to find reliable methods to accurately determine the amount of wheat protein in food. The objective of this study was to compare the quantitative detection of wheat protein in model sausages by ELISA and immunohistochemical methods. Immunohistochemistry was combined with stereology to achieve quantitative results. High correlation between addition of wheat protein and compared methods was confirmed. For ELISA method the determined values were r = 0.98, P < 0.01; for stereologythe determined values were r = 0.94, P < 0.01. Although ELISA is an accredited method, it was not reliable, unlike immunohistochemical methods (stereology SD = 3.1).
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
GM - Potravinářství
OECD FORD obor
—
Návaznosti výsledku
Projekt
—
Návaznosti
N - Vyzkumna aktivita podporovana z neverejnych zdroju
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
ACTA VETERINARIA BRNO
ISSN
0001-7213
e-ISSN
—
Svazek periodika
83
Číslo periodika v rámci svazku
Suppl 10
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
6
Strana od-do
71-76
Kód UT WoS článku
000354505500012
EID výsledku v databázi Scopus
—