Simultaneous detection of peanut and hazelnut allergens in food matrices using multiplex PCR method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F15%3A%230001353" target="_blank" >RIV/00027162:_____/15:#0001353 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.2754/avb201483S10S77" target="_blank" >http://dx.doi.org/10.2754/avb201483S10S77</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201483S10S77" target="_blank" >10.2754/avb201483S10S77</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Simultaneous detection of peanut and hazelnut allergens in food matrices using multiplex PCR method

Popis výsledku v původním jazyce

Multiplex PCR analysis for the detection of two targeting segments of genes coding major food protein allergens as peanut (Arachis hypogaea) Ara h 1 gene and hazelnut (Corylus avellana) Cor a 1 gene was developed. Two sets of primers were designed and tested to their specificity on a broad range of ingredients. The identity of amplicons (Ara h 1- 180 bp, Cor a 1 - 258 bp) by sequencing and alignment of sequences with sequences deposited in Genbank was confirmed. When testing the specificity of designedprimer pairs on a spectrum of food ingredients, no cross reactions were detected. A potential inhibition of PCR reaction was eliminated using the universal plant primers of chloroplast gene 124 bp for the plant matrices confirmation. The intrinsic detection limit was 10 pg.ml-1 and the practical detection limit was 0.001% w/w (10 mg.kg-1) for both peanuts and hazelnuts. The method was applied to the investigation of 60 commercial food samples. The developed multiplex PCR method is cheap,

Název v anglickém jazyce

Simultaneous detection of peanut and hazelnut allergens in food matrices using multiplex PCR method

Popis výsledku anglicky

Multiplex PCR analysis for the detection of two targeting segments of genes coding major food protein allergens as peanut (Arachis hypogaea) Ara h 1 gene and hazelnut (Corylus avellana) Cor a 1 gene was developed. Two sets of primers were designed and tested to their specificity on a broad range of ingredients. The identity of amplicons (Ara h 1- 180 bp, Cor a 1 - 258 bp) by sequencing and alignment of sequences with sequences deposited in Genbank was confirmed. When testing the specificity of designedprimer pairs on a spectrum of food ingredients, no cross reactions were detected. A potential inhibition of PCR reaction was eliminated using the universal plant primers of chloroplast gene 124 bp for the plant matrices confirmation. The intrinsic detection limit was 10 pg.ml-1 and the practical detection limit was 0.001% w/w (10 mg.kg-1) for both peanuts and hazelnuts. The method was applied to the investigation of 60 commercial food samples. The developed multiplex PCR method is cheap,

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/LO1218" target="_blank" >LO1218: Zdravé zvíře jako zdroj zdravé potraviny</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACTA VETERINARIA BRNO

ISSN

0001-7213

e-ISSN

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Svazek periodika

84

Číslo periodika v rámci svazku

83

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

77-83

Kód UT WoS článku

000354505500013

EID výsledku v databázi Scopus

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