Effect of dietary fish oil on expression of liver genes controlling cholesterol homeostasis: comparison of two animal models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F16%3AN0000147" target="_blank" >RIV/00027162:_____/16:N0000147 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/16:43910214

Výsledek na webu

<a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Effect of dietary fish oil on expression of liver genes controlling cholesterol homeostasis: comparison of two animal models

Popis výsledku v původním jazyce

The aim of this study was to compare the effect of the fish oil, respectively its main component, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the expression of genes PPAR alpha, SREBP-2, Insig-1, Hmgcr and Ldlr which control cholesterol homeostasis in the liver of rats (Wistar Albino; n = 32) and pigs (Large White x Landrace; n = 32). Rats and pigs were randomly assigned into two groups of 16 animals and fed ten weeks by the diet with either 2.5% of fish oil (group F; source of eicosapentaenoic and docosahexaenoic acid, EPA+DHA) or 2.5% of palm oil (group P; high content of saturated fatty acids; control group). Dietary fish oil relative to palm oil increased PPAR alpha and SREBP-2 gene expression much strongly (P<0.01) in the pig liver in comparison with the rat liver, but expression of Insig-1 and Hmgcr genes in the liver of the F-pigs relative to the expression of these genes in the liver of the P-pigs was substantially lower (P<0.01 and P<0.05, respectively) as compared to rats.

Název v anglickém jazyce

Effect of dietary fish oil on expression of liver genes controlling cholesterol homeostasis: comparison of two animal models

Popis výsledku anglicky

The aim of this study was to compare the effect of the fish oil, respectively its main component, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the expression of genes PPAR alpha, SREBP-2, Insig-1, Hmgcr and Ldlr which control cholesterol homeostasis in the liver of rats (Wistar Albino; n = 32) and pigs (Large White x Landrace; n = 32). Rats and pigs were randomly assigned into two groups of 16 animals and fed ten weeks by the diet with either 2.5% of fish oil (group F; source of eicosapentaenoic and docosahexaenoic acid, EPA+DHA) or 2.5% of palm oil (group P; high content of saturated fatty acids; control group). Dietary fish oil relative to palm oil increased PPAR alpha and SREBP-2 gene expression much strongly (P<0.01) in the pig liver in comparison with the rat liver, but expression of Insig-1 and Hmgcr genes in the liver of the F-pigs relative to the expression of these genes in the liver of the P-pigs was substantially lower (P<0.01 and P<0.05, respectively) as compared to rats.

Druh

D - Stať ve sborníku

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

MendelNet 2016

ISBN

978-80-7509-443-8

ISSN

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e-ISSN

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Počet stran výsledku

5

Strana od-do

841-845

Název nakladatele

Mendelova Univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

9. 11. 2016

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000392968500151