Detection and identification of yeast in milk from cows with subclinical mastitis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000148" target="_blank" >RIV/00027162:_____/18:N0000148 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.eavld2018.org/images/files/EAVLD_2018-Abstract_book.pdf" target="_blank" >http://www.eavld2018.org/images/files/EAVLD_2018-Abstract_book.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection and identification of yeast in milk from cows with subclinical mastitis

Popis výsledku v původním jazyce

5th Congress of the European association of veterinary laboratory diagnosticians, Brusel, Belgie, 14-17.10.2018 - lecture. Introduction: Mastitis are among the most important and most common diseases of dairy cows. Although, most control measurements are focused on detection and elimination bacterial pathogens, there is growing interest in non-bacterial agents causing mastitis such as yeast. Material and methods: In total, 910 randomly selected milk samples from cow with subclinical mastitis were collected from 17 dairy farms in the Czech Republic. Samples were cultivated on Sabouraud agar with glucose and chloramphenicol and DRBC agar. Isolates were identified by using biochemical assay and by molecular analysis (sequencing of D1/D2 and ITS region). Results: Out of 910 milk samples, 67 yeast isolates from 64 (7.0%) milk samples were obtained. Candida spp. and Trichosporon spp. were most frequently identified species. Nevertheless, biochemical analysis was usually not able to differentiate individual species in comparison to molecular methods. Using molecular identification, 54% (36) of isolates belong to genus Candida with the most common species C. krusei, C. kefyr and C. famata. Trichosporon sp. with the most common species T. cutaneum and T. lactis was identified in 19% (13) of isolates. Discussion: In different countries, the frequency of yeast isolation from cow varies considerably, with reported rates less than 2% in countries with good hygiene condition and with elevated rates more than 15% in countries with poor hygienic condition. It is demonstrated that Candida spp. are the most frequently isolated species, which is in agreement to our study. In addition, C. albicans common human pathogen was not isolated in our study.

Název v anglickém jazyce

Detection and identification of yeast in milk from cows with subclinical mastitis

Popis výsledku anglicky

5th Congress of the European association of veterinary laboratory diagnosticians, Brusel, Belgie, 14-17.10.2018 - lecture. Introduction: Mastitis are among the most important and most common diseases of dairy cows. Although, most control measurements are focused on detection and elimination bacterial pathogens, there is growing interest in non-bacterial agents causing mastitis such as yeast. Material and methods: In total, 910 randomly selected milk samples from cow with subclinical mastitis were collected from 17 dairy farms in the Czech Republic. Samples were cultivated on Sabouraud agar with glucose and chloramphenicol and DRBC agar. Isolates were identified by using biochemical assay and by molecular analysis (sequencing of D1/D2 and ITS region). Results: Out of 910 milk samples, 67 yeast isolates from 64 (7.0%) milk samples were obtained. Candida spp. and Trichosporon spp. were most frequently identified species. Nevertheless, biochemical analysis was usually not able to differentiate individual species in comparison to molecular methods. Using molecular identification, 54% (36) of isolates belong to genus Candida with the most common species C. krusei, C. kefyr and C. famata. Trichosporon sp. with the most common species T. cutaneum and T. lactis was identified in 19% (13) of isolates. Discussion: In different countries, the frequency of yeast isolation from cow varies considerably, with reported rates less than 2% in countries with good hygiene condition and with elevated rates more than 15% in countries with poor hygienic condition. It is demonstrated that Candida spp. are the most frequently isolated species, which is in agreement to our study. In addition, C. albicans common human pathogen was not isolated in our study.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40201 - Animal and dairy science; (Animal biotechnology to be 4.4)

Projekt

<a href="/cs/project/QJ1210301" target="_blank" >QJ1210301: Výzkum, nové produkty a služby pro vytvoření centra prevence, detekce a podpory léčby mastitid.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů