Identification of canned tuna Thunnus albacares and Thunnus alalunga by real-time PCR method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000196" target="_blank" >RIV/00027162:_____/18:N0000196 - isvavai.cz</a>

Výsledek na webu

<a href="https://food-technology.nutritionalconference.com" target="_blank" >https://food-technology.nutritionalconference.com</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of canned tuna Thunnus albacares and Thunnus alalunga by real-time PCR method

Popis výsledku v původním jazyce

The resulting abstract from the conference is published in the abstracts proceedings organized by the World Congress on Food and Nutrition in Dubai, 2018. Food authenticity testing is one of the major challenges facing the food safety authorities. Pursuant to Council Regulation (EEC) No. 1536/1992, tunas are classified into true tunas (Thunnus thynnus, T. albacares, T. alalunga, T. obesus etc., Euthynnus sp. (Katsuwonus pelamis) or pseudo-tunas, i.e. bonito (Sarda sp., Euthynnus sp. (except Euthynnus pelamis) and Auxis sp.). Tuna fish are often sold as a heat-processed canned products at the market. Different quality and price of tuna species can lead the producer to the adulteration/fraud. The main difficulties in developing of method for these fish species identification is high similarity of DNA sequences among close relative fish species. All complete mitochondrial DNA sequences of yellowfin tuna (Thunnus albacares) and albacore tuna (Thunnus alalunga) were compared to all other mitochondrial DNA sequences of tuna fish saved in GeneBank. The variable sequences inside species were detected and deleted from another comparison. The most variable regions within species were determined and primers and probes were designed in this region for the species specific DNA amplification of yellowfin tuna and albacore tuna. Moreover to check the content of amplifiable DNA of fish (namely tuna) in the sample, the primers and probe of mitochondrial 12S rRNA gene in the region of conservative sequence for fish were designed. Real time PCR methods were verified investigating of 25 samples of canned tuna with the declared content of yellowfin tuna or albacore tuna from the market. All samples contained tuna species in spite of the declaration.

Název v anglickém jazyce

Identification of canned tuna Thunnus albacares and Thunnus alalunga by real-time PCR method

Popis výsledku anglicky

The resulting abstract from the conference is published in the abstracts proceedings organized by the World Congress on Food and Nutrition in Dubai, 2018. Food authenticity testing is one of the major challenges facing the food safety authorities. Pursuant to Council Regulation (EEC) No. 1536/1992, tunas are classified into true tunas (Thunnus thynnus, T. albacares, T. alalunga, T. obesus etc., Euthynnus sp. (Katsuwonus pelamis) or pseudo-tunas, i.e. bonito (Sarda sp., Euthynnus sp. (except Euthynnus pelamis) and Auxis sp.). Tuna fish are often sold as a heat-processed canned products at the market. Different quality and price of tuna species can lead the producer to the adulteration/fraud. The main difficulties in developing of method for these fish species identification is high similarity of DNA sequences among close relative fish species. All complete mitochondrial DNA sequences of yellowfin tuna (Thunnus albacares) and albacore tuna (Thunnus alalunga) were compared to all other mitochondrial DNA sequences of tuna fish saved in GeneBank. The variable sequences inside species were detected and deleted from another comparison. The most variable regions within species were determined and primers and probes were designed in this region for the species specific DNA amplification of yellowfin tuna and albacore tuna. Moreover to check the content of amplifiable DNA of fish (namely tuna) in the sample, the primers and probe of mitochondrial 12S rRNA gene in the region of conservative sequence for fish were designed. Real time PCR methods were verified investigating of 25 samples of canned tuna with the declared content of yellowfin tuna or albacore tuna from the market. All samples contained tuna species in spite of the declaration.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/QJ1530272" target="_blank" >QJ1530272: Komplexní strategie pro efektivní odhalování falšování potravin v řetězci (prvo)výroba - spotřebitel</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů