Size-segregated urban aerosol characterization by electron microscopy and dynamic light scattering and influence of sample preparation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F18%3AN0000231" target="_blank" >RIV/00027162:_____/18:N0000231 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081731:_____/18:00494587 RIV/00216208:11310/18:10378428

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S135223101830075X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S135223101830075X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.atmosenv.2018.02.004" target="_blank" >10.1016/j.atmosenv.2018.02.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Size-segregated urban aerosol characterization by electron microscopy and dynamic light scattering and influence of sample preparation

Popis výsledku v původním jazyce

Size-segregated particulate matter (PM) is frequently used in chemical and toxicological studies. Nevertheless, toxicological in vitro studies working with the whole particles often lack a proper evaluation of PM real size distribution and characterization of agglomeration under the experimental conditions. In this study, changes in particle size distributions during the PM sample manipulation and also semiquantitative elemental composition of single particles were evaluated. Coarse (1–10 μm), upper accumulation (0.5–1 μm), lower accumulation (0.17–0.5 μm), and ultrafine (< 0.17 μm) PM fractions were collected by high volume cascade impactor in Prague city center. Particles were examined using electron microscopy and their elemental composition was determined by energy dispersive X-ray spectroscopy. Larger or smaller particles, not corresponding to the impaction cut points, were found in all fractions, as they occur in agglomerates and are impacted according to their aerodynamic diameter. Elemental composition of particles in size-segregated fractions varied significantly. Nssoot occurred in all size fractions. Metallic nanospheres were found in accumulation fractions, but not in ultrafine fraction where ns-soot, carbonaceous particles, and inorganic salts were identified. Dynamic light scattering was used to measure particle size distribution in water and in cell culture media. PM suspension of lower accumulation fraction in water agglomerated after freezing/thawing the sample, and the agglomerates were disrupted by subsequent sonication. Ultrafine fraction did not agglomerate after freezing/thawing the sample. Both lower accumulation and ultrafine fractions were stable in cell culture media with fetal bovine serum, while high agglomeration occurred in media without fetal bovine serum as measured during 24 h.

Název v anglickém jazyce

Size-segregated urban aerosol characterization by electron microscopy and dynamic light scattering and influence of sample preparation

Popis výsledku anglicky

Size-segregated particulate matter (PM) is frequently used in chemical and toxicological studies. Nevertheless, toxicological in vitro studies working with the whole particles often lack a proper evaluation of PM real size distribution and characterization of agglomeration under the experimental conditions. In this study, changes in particle size distributions during the PM sample manipulation and also semiquantitative elemental composition of single particles were evaluated. Coarse (1–10 μm), upper accumulation (0.5–1 μm), lower accumulation (0.17–0.5 μm), and ultrafine (< 0.17 μm) PM fractions were collected by high volume cascade impactor in Prague city center. Particles were examined using electron microscopy and their elemental composition was determined by energy dispersive X-ray spectroscopy. Larger or smaller particles, not corresponding to the impaction cut points, were found in all fractions, as they occur in agglomerates and are impacted according to their aerodynamic diameter. Elemental composition of particles in size-segregated fractions varied significantly. Nssoot occurred in all size fractions. Metallic nanospheres were found in accumulation fractions, but not in ultrafine fraction where ns-soot, carbonaceous particles, and inorganic salts were identified. Dynamic light scattering was used to measure particle size distribution in water and in cell culture media. PM suspension of lower accumulation fraction in water agglomerated after freezing/thawing the sample, and the agglomerates were disrupted by subsequent sonication. Ultrafine fraction did not agglomerate after freezing/thawing the sample. Both lower accumulation and ultrafine fractions were stable in cell culture media with fetal bovine serum, while high agglomeration occurred in media without fetal bovine serum as measured during 24 h.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30304 - Public and environmental health

Projekt

<a href="/cs/project/GBP503%2F12%2FG147" target="_blank" >GBP503/12/G147: Centrum studií toxických vlastností nanočástic</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Atmospheric Environment

ISSN

1352-2310

e-ISSN

1873-2844

Svazek periodika

178

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

181-190

Kód UT WoS článku

000428104200020

EID výsledku v databázi Scopus

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