Monitoring of selected zoonotic parasites in fruits and vegetables

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000048" target="_blank" >RIV/00027162:_____/19:N0000048 - isvavai.cz</a>

Výsledek na webu

<a href="http://hygiena-alimentorum.uvlf.sk/program.php" target="_blank" >http://hygiena-alimentorum.uvlf.sk/program.php</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Monitoring of selected zoonotic parasites in fruits and vegetables

Popis výsledku v původním jazyce

Hygiena Alimentorum, 15.-17.5.2019, Štrbské Pleso (Slovakia) – lecture and text in peer-reviewed proceedings. Fresh vegetables, fruits, herbs and sprout seeds are usually prepared and consumed raw, without cooking or heating that leads to a risk of foodborne infection. Besides bacterial and viral agents these infections may also be caused by parasitic agents. These parasitic infections are often referred as neglected, although such infections may have had serious consequences for human health. Our goal was to assess the parasitic contamination of vegetables, fruits, herbs and sprout seeds, either raw or frozen. Specifically, the work focused on the detection of Cryptosporidium parvum (CP), Giardia intestinalis (GI) and Toxoplasma gondii (TG). During five-year monitoring, almost 1100 samples of fresh or frozen fruits and vegetables, herbs and sprout seeds were collected and analyzed. Samples originated from fields, fruit farms and markets from Czech Republic but also from other EU countries. We tested the samples using qPCR assay targeting hsp70 (CP), β – giardin gene (GI) and B1 and 529rep loci for TG. Our results revealed that there were 2.3% positive samples for CP. 1.7% of the samples were found positive for GI. DNA of TG was detected in 8.7% of samples. Fresh vegetables (lettuce, cucumber, carrot) and fresh strawberries were the most contaminated samples with these agents. Although the positivity of the samples was not high the occurrence of the parasitic stages is still relevant for human health and can cause clinical illness.

Název v anglickém jazyce

Monitoring of selected zoonotic parasites in fruits and vegetables

Popis výsledku anglicky

Hygiena Alimentorum, 15.-17.5.2019, Štrbské Pleso (Slovakia) – lecture and text in peer-reviewed proceedings. Fresh vegetables, fruits, herbs and sprout seeds are usually prepared and consumed raw, without cooking or heating that leads to a risk of foodborne infection. Besides bacterial and viral agents these infections may also be caused by parasitic agents. These parasitic infections are often referred as neglected, although such infections may have had serious consequences for human health. Our goal was to assess the parasitic contamination of vegetables, fruits, herbs and sprout seeds, either raw or frozen. Specifically, the work focused on the detection of Cryptosporidium parvum (CP), Giardia intestinalis (GI) and Toxoplasma gondii (TG). During five-year monitoring, almost 1100 samples of fresh or frozen fruits and vegetables, herbs and sprout seeds were collected and analyzed. Samples originated from fields, fruit farms and markets from Czech Republic but also from other EU countries. We tested the samples using qPCR assay targeting hsp70 (CP), β – giardin gene (GI) and B1 and 529rep loci for TG. Our results revealed that there were 2.3% positive samples for CP. 1.7% of the samples were found positive for GI. DNA of TG was detected in 8.7% of samples. Fresh vegetables (lettuce, cucumber, carrot) and fresh strawberries were the most contaminated samples with these agents. Although the positivity of the samples was not high the occurrence of the parasitic stages is still relevant for human health and can cause clinical illness.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30310 - Parasitology

Projekt

<a href="/cs/project/QK1810212" target="_blank" >QK1810212: Rychlé, komplexní a multiplexní metody pro simultánní detekci původců alimentárních onemocnění v potravinách živočišného a rostlinného původu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů