Presence of Giardia intestinalis in ready to eat foods of plant origin

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000071" target="_blank" >RIV/00027162:_____/19:N0000071 - isvavai.cz</a>

Výsledek na webu

<a href="https://en.rouentourisme.com/7thigcc/" target="_blank" >https://en.rouentourisme.com/7thigcc/</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Presence of Giardia intestinalis in ready to eat foods of plant origin

Popis výsledku v původním jazyce

7th International Giardia and Cryptosporidium conference 23.-26.6.2019 Rouen, France – poster. The protozoan parasites (Giardia intestinalis, Cryptosporidium parvum, Toxoplasma gondii) are pathogens that pose significant risks to public health worldwide. Water for drinking, washing or irrigation is one of the source of parasites (or their life stages) for foodstuff, generally. Especially in ready to eat foodstuff (RTE) which are not thermally treated, the presence of parasites/life stages of any of their amounts is unacceptable. The aim of the study was to monitor of RTE foodstuff (herbs, friut and vegetable) for the presence of Giardia intestinalis (GI). During five-year monitoring of fresh or frozen fruit and vegetable, herbs and sprout seeds was collected 1088 samples. Samples originated from field, fruit farms or markets. Country of origin samples was not only Czech Republic but also other EU countries (especially samples from market were not all from Czech Republic). By a newly developed DNA isolation and GI-specific real time PCR (targeting β – giardin gene) was processed one hundred grams per sample. None sample of sprout seeds (31 tested samples) was found to be positive for GL DNA presence. Presence of GL DNA in amounts 1-10 organism/gram was found in one sample of herbs (227 tested samples), in 15 samples of vegetable (574 tested samples) and in two samples of fruits (249 tested samples) was detected.

Název v anglickém jazyce

Presence of Giardia intestinalis in ready to eat foods of plant origin

Popis výsledku anglicky

7th International Giardia and Cryptosporidium conference 23.-26.6.2019 Rouen, France – poster. The protozoan parasites (Giardia intestinalis, Cryptosporidium parvum, Toxoplasma gondii) are pathogens that pose significant risks to public health worldwide. Water for drinking, washing or irrigation is one of the source of parasites (or their life stages) for foodstuff, generally. Especially in ready to eat foodstuff (RTE) which are not thermally treated, the presence of parasites/life stages of any of their amounts is unacceptable. The aim of the study was to monitor of RTE foodstuff (herbs, friut and vegetable) for the presence of Giardia intestinalis (GI). During five-year monitoring of fresh or frozen fruit and vegetable, herbs and sprout seeds was collected 1088 samples. Samples originated from field, fruit farms or markets. Country of origin samples was not only Czech Republic but also other EU countries (especially samples from market were not all from Czech Republic). By a newly developed DNA isolation and GI-specific real time PCR (targeting β – giardin gene) was processed one hundred grams per sample. None sample of sprout seeds (31 tested samples) was found to be positive for GL DNA presence. Presence of GL DNA in amounts 1-10 organism/gram was found in one sample of herbs (227 tested samples), in 15 samples of vegetable (574 tested samples) and in two samples of fruits (249 tested samples) was detected.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30310 - Parasitology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů