In vitro inhibition of Escherichia coli by supernatant of Bacillus coagulans PP01

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000049" target="_blank" >RIV/00027162:_____/19:N0000049 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vitro inhibition of Escherichia coli by supernatant of Bacillus coagulans PP01

Popis výsledku v původním jazyce

3rd E. coli and Mucosa Immune System, 2.-5.6.2019, Ghent, Belgium. Escherichia coli is a part of the intestinal microflora. Due to the formation of adhesive molecules and toxins, some strains can cause intestinal infections in humans and animals, incl. post-weaning diarrhea in piglets. One of the way how to try to manage the problem is to use probiotic culture which is able to inhibit the growth of Escherichia coli. Aim of the study was to test ability of supernatant of Bacillus coagulans PP01 to inhibit Escherichia coli in vitro. Selected strain of Bacillus coagulans PP01 was positive for enzymatic activity of leucine-, valine- and cystine-arylaminase, naphthol-AS-BI-physphohydrolase, α-glucosidase, β-glucosidase and N-Acetyl-β-glucosaminidase. Ability of supernatant to inhibit growth of E.coli was tested by modification of agar diffuse agar. It was found that the supernatant was able to down-regulate E.coli in a dose-dependent manner. Mean inhibition zone diameter ranged from 0 to 16 from 0.5 to 10 mg of supernatant, respectively. In the experiment, ability of supernatant of Bacillus coagulans PP01 to inhibit Escherichia coli growth in vitro was proven.

Název v anglickém jazyce

In vitro inhibition of Escherichia coli by supernatant of Bacillus coagulans PP01

Popis výsledku anglicky

3rd E. coli and Mucosa Immune System, 2.-5.6.2019, Ghent, Belgium. Escherichia coli is a part of the intestinal microflora. Due to the formation of adhesive molecules and toxins, some strains can cause intestinal infections in humans and animals, incl. post-weaning diarrhea in piglets. One of the way how to try to manage the problem is to use probiotic culture which is able to inhibit the growth of Escherichia coli. Aim of the study was to test ability of supernatant of Bacillus coagulans PP01 to inhibit Escherichia coli in vitro. Selected strain of Bacillus coagulans PP01 was positive for enzymatic activity of leucine-, valine- and cystine-arylaminase, naphthol-AS-BI-physphohydrolase, α-glucosidase, β-glucosidase and N-Acetyl-β-glucosaminidase. Ability of supernatant to inhibit growth of E.coli was tested by modification of agar diffuse agar. It was found that the supernatant was able to down-regulate E.coli in a dose-dependent manner. Mean inhibition zone diameter ranged from 0 to 16 from 0.5 to 10 mg of supernatant, respectively. In the experiment, ability of supernatant of Bacillus coagulans PP01 to inhibit Escherichia coli growth in vitro was proven.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1810463" target="_blank" >QK1810463: Vývoj nové formy probiotické superabsorpční podestýlky s následným využitím pro zadržení dešťové vody v půdě</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů