Prediction of persistence of Listeria monocytogenes ST451 in a rabbit processing plant in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000072" target="_blank" >RIV/00027162:_____/19:N0000072 - isvavai.cz</a>

Výsledek na webu

<a href="https://jfoodprotection.org/doi/abs/10.4315/0362-028X.JFP-19-030" target="_blank" >https://jfoodprotection.org/doi/abs/10.4315/0362-028X.JFP-19-030</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4315/0362-028X.JFP-19-030" target="_blank" >10.4315/0362-028X.JFP-19-030</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Prediction of persistence of Listeria monocytogenes ST451 in a rabbit processing plant in the Czech Republic

Popis výsledku v původním jazyce

The study was focused on characterization of the genetic diversity of L. monocytogenes isolated from packed fresh rabbit meat obtained from one producer via retail outlets. The partial aim was to compare the characteristics of a suspect persistent strain with strains originating in human cases. The occurrence of L. monocytogenes in vacuum packed rabbit meat was monitored during the period of 2013-2016. All strains were characterized by serotyping, multilocus sequence typing (MLST) and macrorestriction analysis. Selected strains, which represented each year, were analysed using the WGS (whole genome sequencing) method. L. monocytogenes was detected in 38% (21/56) of originally packed rabbit meat samples from one food producer during the whole monitored period. All strains showed the identical serotype (1/2a), AscI/ApaI pulsotype (735/2) and sequence type (ST451). The clonal similarity of strains from rabbit meat was also confirmed on the basis of core genome MLST. This fact suggests the occurrence of a suspect persistent strain in the meat processing plant. Results of cgMLST enabled us to unambiguously exclude rabbit meat as a source of listeriosis in humans caused by the indistinguishable AscI/ApaI pulsotype, although the strains of ST451 carried all genes important for the pathogenicity of L. monocytogenes. No specific genes which may be associated with its persistence in food processing environments were detected among the tested strains of ST451.

Název v anglickém jazyce

Prediction of persistence of Listeria monocytogenes ST451 in a rabbit processing plant in the Czech Republic

Popis výsledku anglicky

The study was focused on characterization of the genetic diversity of L. monocytogenes isolated from packed fresh rabbit meat obtained from one producer via retail outlets. The partial aim was to compare the characteristics of a suspect persistent strain with strains originating in human cases. The occurrence of L. monocytogenes in vacuum packed rabbit meat was monitored during the period of 2013-2016. All strains were characterized by serotyping, multilocus sequence typing (MLST) and macrorestriction analysis. Selected strains, which represented each year, were analysed using the WGS (whole genome sequencing) method. L. monocytogenes was detected in 38% (21/56) of originally packed rabbit meat samples from one food producer during the whole monitored period. All strains showed the identical serotype (1/2a), AscI/ApaI pulsotype (735/2) and sequence type (ST451). The clonal similarity of strains from rabbit meat was also confirmed on the basis of core genome MLST. This fact suggests the occurrence of a suspect persistent strain in the meat processing plant. Results of cgMLST enabled us to unambiguously exclude rabbit meat as a source of listeriosis in humans caused by the indistinguishable AscI/ApaI pulsotype, although the strains of ST451 carried all genes important for the pathogenicity of L. monocytogenes. No specific genes which may be associated with its persistence in food processing environments were detected among the tested strains of ST451.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21101 - Food and beverages

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Protection

ISSN

0362-028X

e-ISSN

1944-9097

Svazek periodika

82

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

1350-1356

Kód UT WoS článku

000477777500011

EID výsledku v databázi Scopus

2-s2.0-85070729028