Sperm and testicular measurements and sperm cryopreservation in the giraffe (Giraffa)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000294" target="_blank" >RIV/00027162:_____/19:N0000294 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s10344-019-1257-7" target="_blank" >https://link.springer.com/article/10.1007/s10344-019-1257-7</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Sperm and testicular measurements and sperm cryopreservation in the giraffe (Giraffa)

Popis výsledku v původním jazyce

Giraffes are numbered amongst vulnerable mammalian species which are likely to become endangered due to the ongoing population decline. In endangered species, the ex-situ biodiversity conservation efforts involve captive breeding, genome resource banking and artificial reproduction using cryopreserved gametes. Determination of the sperm standards and the sensitivity of spermatozoa to freezing are crucial points in such activities. We analysed epididymal sperm retrieved from three captive bred giraffe males (two G. reticulata, one G. camelopardalis rothschildi) and tested the sensitivity of giraffe sperm to cryopreservation. The basic testicular and sperm morphometric measurements, the sperm functional and morphological assessment and the sperm chromatin structure analysis revealed inter-individual differences. The epididymal sperm quality of males G2 and G3 was low, probably due to the long-term health problems in G2, sexual immaturity in G3, and longer period between the death/neutering and the sperm retrieval in both animals. Epididymal sperm of male G1 were subjected to cryopreservation in TRIS-fructose-citric acid media with egg yolk. The tested cryopreservation method was approved as suitable for banking giraffe spermatozoa. In the case of endangered species, euthanized and neutered animals can serve as a source of gametes for genome resource banking.

Název v anglickém jazyce

Sperm and testicular measurements and sperm cryopreservation in the giraffe (Giraffa)

Popis výsledku anglicky

Giraffes are numbered amongst vulnerable mammalian species which are likely to become endangered due to the ongoing population decline. In endangered species, the ex-situ biodiversity conservation efforts involve captive breeding, genome resource banking and artificial reproduction using cryopreserved gametes. Determination of the sperm standards and the sensitivity of spermatozoa to freezing are crucial points in such activities. We analysed epididymal sperm retrieved from three captive bred giraffe males (two G. reticulata, one G. camelopardalis rothschildi) and tested the sensitivity of giraffe sperm to cryopreservation. The basic testicular and sperm morphometric measurements, the sperm functional and morphological assessment and the sperm chromatin structure analysis revealed inter-individual differences. The epididymal sperm quality of males G2 and G3 was low, probably due to the long-term health problems in G2, sexual immaturity in G3, and longer period between the death/neutering and the sperm retrieval in both animals. Epididymal sperm of male G1 were subjected to cryopreservation in TRIS-fructose-citric acid media with egg yolk. The tested cryopreservation method was approved as suitable for banking giraffe spermatozoa. In the case of endangered species, euthanized and neutered animals can serve as a source of gametes for genome resource banking.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10603 - Genetics and heredity (medical genetics to be 3)

Projekt

<a href="/cs/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

European Journal of Wildlife Research

ISSN

1612-4642

e-ISSN

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Svazek periodika

65

Číslo periodika v rámci svazku

FEB 2019

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

16

Kód UT WoS článku

000457522400002

EID výsledku v databázi Scopus

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