Systematic Culturomics Shows that Half of Chicken Caecal Microbiota Members can be Grown in Vitro Except for Two Lineages of Clostridiales and a Single Lineage of Bacteroidetes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F19%3AN0000335" target="_blank" >RIV/00027162:_____/19:N0000335 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/19:43877837 RIV/62157124:16810/19:43877837

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pubmed/31661802" target="_blank" >https://www.ncbi.nlm.nih.gov/pubmed/31661802</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms7110496" target="_blank" >10.3390/microorganisms7110496</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Systematic Culturomics Shows that Half of Chicken Caecal Microbiota Members can be Grown in Vitro Except for Two Lineages of Clostridiales and a Single Lineage of Bacteroidetes

Popis výsledku v původním jazyce

Epidemiological data show that composition of gut microbiota influence host health or disease status, age or even behaviour. However, to confirm epidemiological observation in controlled experiments, pure cultures of gut anaerobes must be obtained. Since this is not a simple task due to large number of bacterial species colonising intestinal tract, in this study we inoculated 174 different culture media with caecal samples from adult hens, and compared microbiota composition in the original caecal samples and in bacterial mass growing in vitro by 16S rRNA sequencing. We observed that 42 % of gut microbiota members could be grown in vitro and since there were some species which we did not culture but for which the culture conditions are known, it is likely that more than half of chicken gut microbiota can be grown in vitro. However, there were two lineages of Clostridiales and a single lineage of Bacteroidetes which were common to caecal microbiota but resistant to culture. Of the most selective culture conditions, nutrient broths supplemented with mono- or disaccharides incl. those present in fruits positively selected for Lactobacillaceae. Addition of bile salts selected for Veillonellaceae and use of YFCA enriched for Desulfovibrionaceae. In addition, Erysipelotrichaceae were positively selected by colistin, trimethoprim, streptomycin and nalidixic acid. Such culture conditions can be therefore used for selective enrichment by desired bacterial species but also indicate specific functions of individual gut microbiota members.

Název v anglickém jazyce

Systematic Culturomics Shows that Half of Chicken Caecal Microbiota Members can be Grown in Vitro Except for Two Lineages of Clostridiales and a Single Lineage of Bacteroidetes

Popis výsledku anglicky

Epidemiological data show that composition of gut microbiota influence host health or disease status, age or even behaviour. However, to confirm epidemiological observation in controlled experiments, pure cultures of gut anaerobes must be obtained. Since this is not a simple task due to large number of bacterial species colonising intestinal tract, in this study we inoculated 174 different culture media with caecal samples from adult hens, and compared microbiota composition in the original caecal samples and in bacterial mass growing in vitro by 16S rRNA sequencing. We observed that 42 % of gut microbiota members could be grown in vitro and since there were some species which we did not culture but for which the culture conditions are known, it is likely that more than half of chicken gut microbiota can be grown in vitro. However, there were two lineages of Clostridiales and a single lineage of Bacteroidetes which were common to caecal microbiota but resistant to culture. Of the most selective culture conditions, nutrient broths supplemented with mono- or disaccharides incl. those present in fruits positively selected for Lactobacillaceae. Addition of bile salts selected for Veillonellaceae and use of YFCA enriched for Desulfovibrionaceae. In addition, Erysipelotrichaceae were positively selected by colistin, trimethoprim, streptomycin and nalidixic acid. Such culture conditions can be therefore used for selective enrichment by desired bacterial species but also indicate specific functions of individual gut microbiota members.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

12

Strana od-do

496

Kód UT WoS článku

000502273600019

EID výsledku v databázi Scopus

—