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Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

Identifikátory výsledku

  • Kód výsledku v IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000065" target="_blank" >RIV/00027162:_____/20:N0000065 - isvavai.cz</a>

  • Výsledek na webu

    <a href="https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-020-00806-9" target="_blank" >https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-020-00806-9</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s13567-020-00806-9" target="_blank" >10.1186/s13567-020-00806-9</a>

Alternativní jazyky

  • Jazyk výsledku

    angličtina

  • Název v původním jazyce

    Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

  • Popis výsledku v původním jazyce

    Encephalitozoonosis is a common infectious disease widely spread among rabbits.Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts. The aim of the study was to describe in detail the spread of theE. cuniculiin a rabbit organism after experimental infection and the host humoral and cellular immune response including cytokine production. For that purpose, healthy immunocompetent rabbits were infected orally in order to simulate the natural route of infection and euthanised at 2, 4, 6 and 8-weeks post-infection. Dissemination ofE. cuniculiin the body of the rabbit was more rapid than previously reported. As early as 2 weeks post-infection,E. cuniculiwas detected using immunohistochemistry not only in the intestine, mesenteric lymph nodes, spleen, liver, kidneys, lungs and heart, but also in nervous tissues, especially in medulla oblongata, cerebellum, and leptomeninges. Based on flow cytometry, no conspicuous changes in lymphocyte subpopulations were detected in the examined lymphoid organs of infected rabbits. Cell-mediated immunity was characterized by ability of both CD4(+)and CD8(+)T cells to proliferate after stimulation with specific antigens. Th1 polarization of immune response with a predominance of IFN-gamma expression was detected in spleen, mesenteric lymph nodes and Peyer's patches. The increased expression of IL-4 and IL-10 mRNA in mixed samples from the small intestine is indicative of balanced control of IFN-gamma, which prevents tissue damage. On the other hand, it can enableE. cuniculito survive and persist in the host organism in a balanced host-parasite relationship. The Th17 immunity lineage seems to play only a minor role inE. cuniculiinfection in rabbits.

  • Název v anglickém jazyce

    Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi

  • Popis výsledku anglicky

    Encephalitozoonosis is a common infectious disease widely spread among rabbits.Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts. The aim of the study was to describe in detail the spread of theE. cuniculiin a rabbit organism after experimental infection and the host humoral and cellular immune response including cytokine production. For that purpose, healthy immunocompetent rabbits were infected orally in order to simulate the natural route of infection and euthanised at 2, 4, 6 and 8-weeks post-infection. Dissemination ofE. cuniculiin the body of the rabbit was more rapid than previously reported. As early as 2 weeks post-infection,E. cuniculiwas detected using immunohistochemistry not only in the intestine, mesenteric lymph nodes, spleen, liver, kidneys, lungs and heart, but also in nervous tissues, especially in medulla oblongata, cerebellum, and leptomeninges. Based on flow cytometry, no conspicuous changes in lymphocyte subpopulations were detected in the examined lymphoid organs of infected rabbits. Cell-mediated immunity was characterized by ability of both CD4(+)and CD8(+)T cells to proliferate after stimulation with specific antigens. Th1 polarization of immune response with a predominance of IFN-gamma expression was detected in spleen, mesenteric lymph nodes and Peyer's patches. The increased expression of IL-4 and IL-10 mRNA in mixed samples from the small intestine is indicative of balanced control of IFN-gamma, which prevents tissue damage. On the other hand, it can enableE. cuniculito survive and persist in the host organism in a balanced host-parasite relationship. The Th17 immunity lineage seems to play only a minor role inE. cuniculiinfection in rabbits.

Klasifikace

  • Druh

    J<sub>imp</sub> - Článek v periodiku v databázi Web of Science

  • CEP obor

  • OECD FORD obor

    40301 - Veterinary science

Návaznosti výsledku

  • Projekt

    Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

  • Návaznosti

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Ostatní

  • Rok uplatnění

    2020

  • Kód důvěrnosti údajů

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Údaje specifické pro druh výsledku

  • Název periodika

    VETERINARY RESEARCH

  • ISSN

    0928-4249

  • e-ISSN

    1297-9716

  • Svazek periodika

    51

  • Číslo periodika v rámci svazku

    1

  • Stát vydavatele periodika

    GB - Spojené království Velké Británie a Severního Irska

  • Počet stran výsledku

    15

  • Strana od-do

    "79"

  • Kód UT WoS článku

    000542952100003

  • EID výsledku v databázi Scopus

    2-s2.0-85086623341