Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F20%3AN0000065" target="_blank" >RIV/00027162:_____/20:N0000065 - isvavai.cz</a>
Výsledek na webu
<a href="https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-020-00806-9" target="_blank" >https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-020-00806-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13567-020-00806-9" target="_blank" >10.1186/s13567-020-00806-9</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi
Popis výsledku v původním jazyce
Encephalitozoonosis is a common infectious disease widely spread among rabbits.Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts. The aim of the study was to describe in detail the spread of theE. cuniculiin a rabbit organism after experimental infection and the host humoral and cellular immune response including cytokine production. For that purpose, healthy immunocompetent rabbits were infected orally in order to simulate the natural route of infection and euthanised at 2, 4, 6 and 8-weeks post-infection. Dissemination ofE. cuniculiin the body of the rabbit was more rapid than previously reported. As early as 2 weeks post-infection,E. cuniculiwas detected using immunohistochemistry not only in the intestine, mesenteric lymph nodes, spleen, liver, kidneys, lungs and heart, but also in nervous tissues, especially in medulla oblongata, cerebellum, and leptomeninges. Based on flow cytometry, no conspicuous changes in lymphocyte subpopulations were detected in the examined lymphoid organs of infected rabbits. Cell-mediated immunity was characterized by ability of both CD4(+)and CD8(+)T cells to proliferate after stimulation with specific antigens. Th1 polarization of immune response with a predominance of IFN-gamma expression was detected in spleen, mesenteric lymph nodes and Peyer's patches. The increased expression of IL-4 and IL-10 mRNA in mixed samples from the small intestine is indicative of balanced control of IFN-gamma, which prevents tissue damage. On the other hand, it can enableE. cuniculito survive and persist in the host organism in a balanced host-parasite relationship. The Th17 immunity lineage seems to play only a minor role inE. cuniculiinfection in rabbits.
Název v anglickém jazyce
Characterization of humoral and cell-mediated immunity in rabbits orally infected with Encephalitozoon cuniculi
Popis výsledku anglicky
Encephalitozoonosis is a common infectious disease widely spread among rabbits.Encephalitozoon cuniculi, is considered as a zoonotic and emerging pathogen capable of infecting both immunocompetent and immunocompromised hosts. The aim of the study was to describe in detail the spread of theE. cuniculiin a rabbit organism after experimental infection and the host humoral and cellular immune response including cytokine production. For that purpose, healthy immunocompetent rabbits were infected orally in order to simulate the natural route of infection and euthanised at 2, 4, 6 and 8-weeks post-infection. Dissemination ofE. cuniculiin the body of the rabbit was more rapid than previously reported. As early as 2 weeks post-infection,E. cuniculiwas detected using immunohistochemistry not only in the intestine, mesenteric lymph nodes, spleen, liver, kidneys, lungs and heart, but also in nervous tissues, especially in medulla oblongata, cerebellum, and leptomeninges. Based on flow cytometry, no conspicuous changes in lymphocyte subpopulations were detected in the examined lymphoid organs of infected rabbits. Cell-mediated immunity was characterized by ability of both CD4(+)and CD8(+)T cells to proliferate after stimulation with specific antigens. Th1 polarization of immune response with a predominance of IFN-gamma expression was detected in spleen, mesenteric lymph nodes and Peyer's patches. The increased expression of IL-4 and IL-10 mRNA in mixed samples from the small intestine is indicative of balanced control of IFN-gamma, which prevents tissue damage. On the other hand, it can enableE. cuniculito survive and persist in the host organism in a balanced host-parasite relationship. The Th17 immunity lineage seems to play only a minor role inE. cuniculiinfection in rabbits.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2020
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
VETERINARY RESEARCH
ISSN
0928-4249
e-ISSN
1297-9716
Svazek periodika
51
Číslo periodika v rámci svazku
1
Stát vydavatele periodika
GB - Spojené království Velké Británie a Severního Irska
Počet stran výsledku
15
Strana od-do
"79"
Kód UT WoS článku
000542952100003
EID výsledku v databázi Scopus
2-s2.0-85086623341