Effect of the vaccination against Shiga toxin 2e in a farm with history of oedema disease, caused by atypical Escherichia coli producing Shiga toxin (STEC)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F22%3AN0000163" target="_blank" >RIV/00027162:_____/22:N0000163 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/22:00128669 RIV/62157124:16170/22:43880387

Výsledek na webu

<a href="https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=36_2022-VETMED" target="_blank" >https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=36_2022-VETMED</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/36/2022-VETMED" target="_blank" >10.17221/36/2022-VETMED</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of the vaccination against Shiga toxin 2e in a farm with history of oedema disease, caused by atypical Escherichia coli producing Shiga toxin (STEC)

Popis výsledku v původním jazyce

Oedema disease (OD) is a disorder occurring in weaned piglets, caused by shigatoxigenic Escherichia coli strains (STEC). These strains typically harbour the stx2e gene and are equipped with fimbrial adhesins F18. Vaccination can be applied as a useful preventive measure but little is known about its efficacy in pigs, in which non-typical E. coli strains have been identified as an ethiological agent causing the OD. Therefore, aim of this study was to assess the effect of a commercially available vaccine application on oedema disease onset and on productive indicators on a farm, with a confirmed history of this disease caused by non-typical STEC. Piglets were separated in two groups. Group A (51 piglets) was vaccinated with Ecoporc Shiga® (Ceva Sante Animale, Libourne, France), group B (62 piglets) was used as control. During the study, weight gain, mortality, individual antibiotic treatment and average daily gain were monitored in all piglets. Fecal samples of the group B were examined for STEC. Isolated strains harbouring stx2e gene and determined by serogroupe were characterized using whole genome sequencing and their effect on Vero cells was assessed. Analysis of the productive indicators revealed significant increase of the body weight of the group A, lower morbidity and mortality of piglets compared to group B. In total, 12 (16.2%) of fecal samples were STEC positive. In isolated strains, serogroups O9 (1) and O100 (1) were confirmed. In two strains serogroup was not specified. No strains harboured genes for typical fimbrial adhesins but genes iha, orfA and orfB associated also with adherence were confirmed. Vero cell assay showed differences in the effect of tested strains on cell proliferation from no effect to high effect in dilution 1:8000. Positive effect of vaccination on monitored productive indicators was confirmed in the study. Furthermore, detail analysis of isolated strains suggests the possible involvement of different types of adhesins than typical F18 fimbriae in pathogenesis of oedema disease.

Název v anglickém jazyce

Effect of the vaccination against Shiga toxin 2e in a farm with history of oedema disease, caused by atypical Escherichia coli producing Shiga toxin (STEC)

Popis výsledku anglicky

Oedema disease (OD) is a disorder occurring in weaned piglets, caused by shigatoxigenic Escherichia coli strains (STEC). These strains typically harbour the stx2e gene and are equipped with fimbrial adhesins F18. Vaccination can be applied as a useful preventive measure but little is known about its efficacy in pigs, in which non-typical E. coli strains have been identified as an ethiological agent causing the OD. Therefore, aim of this study was to assess the effect of a commercially available vaccine application on oedema disease onset and on productive indicators on a farm, with a confirmed history of this disease caused by non-typical STEC. Piglets were separated in two groups. Group A (51 piglets) was vaccinated with Ecoporc Shiga® (Ceva Sante Animale, Libourne, France), group B (62 piglets) was used as control. During the study, weight gain, mortality, individual antibiotic treatment and average daily gain were monitored in all piglets. Fecal samples of the group B were examined for STEC. Isolated strains harbouring stx2e gene and determined by serogroupe were characterized using whole genome sequencing and their effect on Vero cells was assessed. Analysis of the productive indicators revealed significant increase of the body weight of the group A, lower morbidity and mortality of piglets compared to group B. In total, 12 (16.2%) of fecal samples were STEC positive. In isolated strains, serogroups O9 (1) and O100 (1) were confirmed. In two strains serogroup was not specified. No strains harboured genes for typical fimbrial adhesins but genes iha, orfA and orfB associated also with adherence were confirmed. Vero cell assay showed differences in the effect of tested strains on cell proliferation from no effect to high effect in dilution 1:8000. Positive effect of vaccination on monitored productive indicators was confirmed in the study. Furthermore, detail analysis of isolated strains suggests the possible involvement of different types of adhesins than typical F18 fimbriae in pathogenesis of oedema disease.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

1805-9392

Svazek periodika

67

Číslo periodika v rámci svazku

10

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

9

Strana od-do

510-518

Kód UT WoS článku

000852197700001

EID výsledku v databázi Scopus

2-s2.0-85140217879