Characterization of Genotypic and Phenotypic Profile of Lactic Acid Bacteria retrieved from gastrointestinal tract of domestic pigs and wild boars

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F23%3AN0000188" target="_blank" >RIV/00027162:_____/23:N0000188 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Characterization of Genotypic and Phenotypic Profile of Lactic Acid Bacteria retrieved from gastrointestinal tract of domestic pigs and wild boars

Popis výsledku v původním jazyce

The study aims to characterize Lactic Acid Bacteria (LAB) with potentially probiotic features derived from the gastrointestinal tract of domestic pigs and wild boars. In the future, selected isolates with the best probiotic properties should be used as a component of a probiotic mixture to protect domestic piglets from post-weaning diarrhea. Besides the phenotypic testing of LAB isolates, we also performed genotype characterization using Next Generation Sequencing (NGS) by Illumina. Selected representatives were sequenced to find genes encoding beneficial probiotic properties (adhesion-associated genes or CAZy genes). For sequencing data validation, we use tools like Prokka for annotation, Fast-ANI for strain identification, MEGA-X, MAUVE, and Easy Fig for data visualization. Based on appropriate potential probiotic properties, six isolates were selected. These representatives were cultured in special conditions and mixed into probiotic mixtures. After that, we performed two in vivo experiments on post-weaned piglets in experimental stables in our institute. In the first experiment, we tested the functionality of the three different probiotic mixtures (A, B, C) and the efficiency of the two types of starter feed. During 42 day long experiment, we collected data by observing animal increments, nutrient conversion, and hematological parameters. The second in vivo experiment was conceived as infectious. Every group of testing piglets was given an infectious dose of EPEC (enteropathogenic E. coli) to test the functionality of probiotics after infection. After infection, the changes in the clinical state of piglets were observed, and increments and nutrient conversion were measured. As with the non-infectious experiment, blood and stool from piglets at various intervals after infection were collected (data not evaluated yet).

Název v anglickém jazyce

Characterization of Genotypic and Phenotypic Profile of Lactic Acid Bacteria retrieved from gastrointestinal tract of domestic pigs and wild boars

Popis výsledku anglicky

The study aims to characterize Lactic Acid Bacteria (LAB) with potentially probiotic features derived from the gastrointestinal tract of domestic pigs and wild boars. In the future, selected isolates with the best probiotic properties should be used as a component of a probiotic mixture to protect domestic piglets from post-weaning diarrhea. Besides the phenotypic testing of LAB isolates, we also performed genotype characterization using Next Generation Sequencing (NGS) by Illumina. Selected representatives were sequenced to find genes encoding beneficial probiotic properties (adhesion-associated genes or CAZy genes). For sequencing data validation, we use tools like Prokka for annotation, Fast-ANI for strain identification, MEGA-X, MAUVE, and Easy Fig for data visualization. Based on appropriate potential probiotic properties, six isolates were selected. These representatives were cultured in special conditions and mixed into probiotic mixtures. After that, we performed two in vivo experiments on post-weaned piglets in experimental stables in our institute. In the first experiment, we tested the functionality of the three different probiotic mixtures (A, B, C) and the efficiency of the two types of starter feed. During 42 day long experiment, we collected data by observing animal increments, nutrient conversion, and hematological parameters. The second in vivo experiment was conceived as infectious. Every group of testing piglets was given an infectious dose of EPEC (enteropathogenic E. coli) to test the functionality of probiotics after infection. After infection, the changes in the clinical state of piglets were observed, and increments and nutrient conversion were measured. As with the non-infectious experiment, blood and stool from piglets at various intervals after infection were collected (data not evaluated yet).

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1910351" target="_blank" >QK1910351: Divoká a domácí prasata jako zdroj probiotických kultur a vliv technologických postupů přípravy a skladby synbiotického krmiva na jeho efektivitu a funkčnost</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů