Ovine and Caprine Strains of Corynebacterium pseudotuberculosis on Czech Farms—A Comparative Study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000037" target="_blank" >RIV/00027162:_____/24:N0000037 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.mdpi.com/2076-2607/12/5/875" target="_blank" >https://www.mdpi.com/2076-2607/12/5/875</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms12050875" target="_blank" >10.3390/microorganisms12050875</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ovine and Caprine Strains of Corynebacterium pseudotuberculosis on Czech Farms—A Comparative Study

Popis výsledku v původním jazyce

In this study, 33 strains of C. pseudotuberculosis were isolated from sheep and goats suffering from CLA on nine farms in the Czech Republic. All these strains were tested for their antibiotic susceptibility, ability to form a biofilm and resistance to the effects of commonly used disinfectant agents. To better understand the virulence of C. pseudotuberculosis, the genomes of strains were sequenced and comparative genomic analysis was performed with another 123 genomes of the same species, including ovis and equi biovars, downloaded from the NCBI. The genetic determinants for virulence factors responsible for adherence, virulence factors specialized for iron uptake and exotoxin phospholipase D were revealed in every analyzed genome. Carbohy-drate-Active Enzymes were compared revealing the presence of genetic determinants encoding exo-α-sialidase (GH33) and CP40 protein in most of the analyzed genomes. Thirty-three Czech strains of C. pseudotuberculosis were identified as the biovar ovis on the basis of comparative genome analysis. All the compared genomes of the biovar ovis strains were highly similar regardless of their country of origin or host, reflecting their clonal behavior.

Název v anglickém jazyce

Ovine and Caprine Strains of Corynebacterium pseudotuberculosis on Czech Farms—A Comparative Study

Popis výsledku anglicky

In this study, 33 strains of C. pseudotuberculosis were isolated from sheep and goats suffering from CLA on nine farms in the Czech Republic. All these strains were tested for their antibiotic susceptibility, ability to form a biofilm and resistance to the effects of commonly used disinfectant agents. To better understand the virulence of C. pseudotuberculosis, the genomes of strains were sequenced and comparative genomic analysis was performed with another 123 genomes of the same species, including ovis and equi biovars, downloaded from the NCBI. The genetic determinants for virulence factors responsible for adherence, virulence factors specialized for iron uptake and exotoxin phospholipase D were revealed in every analyzed genome. Carbohy-drate-Active Enzymes were compared revealing the presence of genetic determinants encoding exo-α-sialidase (GH33) and CP40 protein in most of the analyzed genomes. Thirty-three Czech strains of C. pseudotuberculosis were identified as the biovar ovis on the basis of comparative genome analysis. All the compared genomes of the biovar ovis strains were highly similar regardless of their country of origin or host, reflecting their clonal behavior.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/QK1910082" target="_blank" >QK1910082: Řešení problematiky výskytu bakteriálních, protozoárních a virových zoonotických agens v chovech malých přežvýkavců</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microorganisms

ISSN

2076-2607

e-ISSN

2076-2607

Svazek periodika

12

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

21

Strana od-do

—

Kód UT WoS článku

001231692700001

EID výsledku v databázi Scopus

2-s2.0-85194368962