Immune response of different carp strains during the koi herpes virus infection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F24%3AN0000223" target="_blank" >RIV/00027162:_____/24:N0000223 - isvavai.cz</a>

Výsledek na webu

<a href="https://eafp.org/the-1st-meeting-of-the-central-and-eastern-european-eafp-branch-2nd-announcement/" target="_blank" >https://eafp.org/the-1st-meeting-of-the-central-and-eastern-european-eafp-branch-2nd-announcement/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Immune response of different carp strains during the koi herpes virus infection

Popis výsledku v původním jazyce

Sebastian, A., Adamek, M., Abdelsalam, E.E.E., Martyrosyan, A., Pojezdal, Ľ., Matějičková, K., Piačková, V., 2024. Immune response of different carp strains during the koi herpes virus infection. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 7. ISBN 978-80-7701-005-4. In recent years, there has been a tremendous increase in knowledge about koi herpesvirus (KHV) and the challenges faced in carp culture, but any new information can support overcoming the virus, which is important for the field, as it can cause a substantial rise in industry loss. Breeding more resistant breeds of carp is one way of minimizing losses due to this disease. The resistance of different strains of carp (koi; Amur wild carp AS; Hungarian cs carp, M2; hybrid of M2 x Amur mirror carp, M2xAL), and grass carp, and their ability to the transfer the infection to a naïve susceptible host have been evaluated in experimental conditions. The infection was induced by cohabitation of tested fish with intraperitoneally infected koi. Samples of blood and tissues were taken every 7th day post-infection (dpi) till 35 dpi. The second phase was initiated by relocating the fish surviving the first phase (according to the strains/species) to aquaria with naïve koi. Samples of gill were taken at 7, 14, and 21 dpi non-lethally, at 28 and 35 dpi lethally together with other tissues. Clinical signs and mortality were observed during both phases. In the first phase, no fish exhibited clinical signs, no mortality was noticed in naïve fish and there was low mortality even among the injected fish. In koi and grass carp, isolated positive 14 dpi. PCR findings in gill were recorded as early as 7 dpi, and in other groups as early as Overall, the highest viral load in the first phase was recorded in koi and the lowest in grass carp. In the second phase, the naïve koi showed more clinical signs from the 7th dpi. The most turbulent course of infection, accompanied by high viral load and mortality, was observed in koi cohabited with M2. Relatively high viral loads were recorded at 28 and 35 dpi in koi cohabited with grass carp. but without clinical signs or mortality. Koi cohabited with AS, and surprisingly also with koi, showed overall the lowest virus copy numbers in gill. Efforts to minimize the number of fish used to comply with the 3Rs and concerns about lack of samples for later sampling due to mortality have resulted in low sampling rates, which may reduce the predictive power of the results. To get more clarity on the immune response of fish depending on the groups, further analysis such as the detection of anti-KHV serum antibodies (ELISA) and analysis of specific immune gene expression level are being carried out and the results will be presented at the conference.

Název v anglickém jazyce

Immune response of different carp strains during the koi herpes virus infection

Popis výsledku anglicky

Sebastian, A., Adamek, M., Abdelsalam, E.E.E., Martyrosyan, A., Pojezdal, Ľ., Matějičková, K., Piačková, V., 2024. Immune response of different carp strains during the koi herpes virus infection. The 1st Meeting of the Central and Eastern European EAFP Branches, Abstract Book, European Association of Fish Pathologists, Křtiny, Czech Republic, 4.–6. 12. 2024, p. 7. ISBN 978-80-7701-005-4. In recent years, there has been a tremendous increase in knowledge about koi herpesvirus (KHV) and the challenges faced in carp culture, but any new information can support overcoming the virus, which is important for the field, as it can cause a substantial rise in industry loss. Breeding more resistant breeds of carp is one way of minimizing losses due to this disease. The resistance of different strains of carp (koi; Amur wild carp AS; Hungarian cs carp, M2; hybrid of M2 x Amur mirror carp, M2xAL), and grass carp, and their ability to the transfer the infection to a naïve susceptible host have been evaluated in experimental conditions. The infection was induced by cohabitation of tested fish with intraperitoneally infected koi. Samples of blood and tissues were taken every 7th day post-infection (dpi) till 35 dpi. The second phase was initiated by relocating the fish surviving the first phase (according to the strains/species) to aquaria with naïve koi. Samples of gill were taken at 7, 14, and 21 dpi non-lethally, at 28 and 35 dpi lethally together with other tissues. Clinical signs and mortality were observed during both phases. In the first phase, no fish exhibited clinical signs, no mortality was noticed in naïve fish and there was low mortality even among the injected fish. In koi and grass carp, isolated positive 14 dpi. PCR findings in gill were recorded as early as 7 dpi, and in other groups as early as Overall, the highest viral load in the first phase was recorded in koi and the lowest in grass carp. In the second phase, the naïve koi showed more clinical signs from the 7th dpi. The most turbulent course of infection, accompanied by high viral load and mortality, was observed in koi cohabited with M2. Relatively high viral loads were recorded at 28 and 35 dpi in koi cohabited with grass carp. but without clinical signs or mortality. Koi cohabited with AS, and surprisingly also with koi, showed overall the lowest virus copy numbers in gill. Efforts to minimize the number of fish used to comply with the 3Rs and concerns about lack of samples for later sampling due to mortality have resulted in low sampling rates, which may reduce the predictive power of the results. To get more clarity on the immune response of fish depending on the groups, further analysis such as the detection of anti-KHV serum antibodies (ELISA) and analysis of specific immune gene expression level are being carried out and the results will be presented at the conference.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

<a href="/cs/project/EF16_019%2F0000869" target="_blank" >EF16_019/0000869: Udržitelná produkce zdravých ryb v různých akvakulturních systémech - PROFISH</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů