Comparison of various methods of ischaemic cardioprotection on vitality of rat heart grafts

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067049" target="_blank" >RIV/00159816:_____/17:00067049 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/17:43875748 RIV/62157124:16370/17:43875748

Výsledek na webu

<a href="http://dx.doi.org/10.2754/avb201786020199" target="_blank" >http://dx.doi.org/10.2754/avb201786020199</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb201786020199" target="_blank" >10.2754/avb201786020199</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of various methods of ischaemic cardioprotection on vitality of rat heart grafts

Popis výsledku v původním jazyce

The aim of the study was to compare 4 modes of ischaemic cardioprotection using continuous prograde autologous blood perfusion of the coronary artery in two hypothermic modes (group A, B) or conventional protection by cooled Hartmann solution (group C) or cooled saline (group D) without perfusion of the graft. Male Wistar rats (n = 24) were divided into four groups (A-D). In groups A (22-25 oC) and B (4-8 oC), blood perfusion rate was 10 ml/h and the graft was placed in a water bath. Groups C, D were initially rinsed with cold (4-8 oC) Hartmann solution (C) and cold saline solution (D), next the graft was placed in a water bath of cold (4-8 oC) Hartmann solution (C) or saline solution (D). The observed time was 30 min after the implemented perfusion (A, B) or initial rinsing (C, D). At 30 min, hearts of all the groups were perfused for 10 min with prograde-autologous arterialized blood at room temperature. At perfusion minute 10, blood was collected for biochemical analysis (sample 1). Sample 2 involved blood from a portable syringe infusion pumps (in parallel with sample 1). Pairwise test differences between samples 1 and 2 were signifcant in all the groups as regards creatine kinase and lactate dehydrogenase values, sampling 1 values being always higher, while cardiac troponin I concentrations were nonsignifcant in the same comparison. The heart rate during the fnal perfusion was identical in all the groups. Our study has demonstrated that all observed cardioprotection modes are useful for experimental heart grafting

Název v anglickém jazyce

Comparison of various methods of ischaemic cardioprotection on vitality of rat heart grafts

Popis výsledku anglicky

The aim of the study was to compare 4 modes of ischaemic cardioprotection using continuous prograde autologous blood perfusion of the coronary artery in two hypothermic modes (group A, B) or conventional protection by cooled Hartmann solution (group C) or cooled saline (group D) without perfusion of the graft. Male Wistar rats (n = 24) were divided into four groups (A-D). In groups A (22-25 oC) and B (4-8 oC), blood perfusion rate was 10 ml/h and the graft was placed in a water bath. Groups C, D were initially rinsed with cold (4-8 oC) Hartmann solution (C) and cold saline solution (D), next the graft was placed in a water bath of cold (4-8 oC) Hartmann solution (C) or saline solution (D). The observed time was 30 min after the implemented perfusion (A, B) or initial rinsing (C, D). At 30 min, hearts of all the groups were perfused for 10 min with prograde-autologous arterialized blood at room temperature. At perfusion minute 10, blood was collected for biochemical analysis (sample 1). Sample 2 involved blood from a portable syringe infusion pumps (in parallel with sample 1). Pairwise test differences between samples 1 and 2 were signifcant in all the groups as regards creatine kinase and lactate dehydrogenase values, sampling 1 values being always higher, while cardiac troponin I concentrations were nonsignifcant in the same comparison. The heart rate during the fnal perfusion was identical in all the groups. Our study has demonstrated that all observed cardioprotection modes are useful for experimental heart grafting

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

86

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

199-206

Kód UT WoS článku

000405584800012

EID výsledku v databázi Scopus

—