Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F22%3A00076330" target="_blank" >RIV/00159816:_____/22:00076330 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216224:14110/22:00128355 RIV/62157124:16270/22:43879998
Výsledek na webu
<a href="https://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=61&article=2271" target="_blank" >https://www.vup.sk/en/index.php?mainID=2&navID=34&version=2&volume=61&article=2271</a>
DOI - Digital Object Identifier
—
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic
Popis výsledku v původním jazyce
Helicobacter pylori is currently found in more than a half of the human population and is the cause of serious gastrointestinal diseases such as gastritis, gastric ulcers or adenocarcinoma. H. pylori infection can be transmitted to humans through contaminated raw materials and food of animal origin. In this study, individual cows' milk samples (n = 77) and individual goats' milk samples (n = 52) from two farms in Moravia (Czech Republic) were analysed. The classical culture method and the nested polymerase chain reaction (nested PCR) method were used for detection of H. pylori. The culture method did not demonstrate the presence of H. pylori in any of the examined samples. Using the nested PCR method, 31 samples (40 %) of raw cows' milk and 30 samples (58 %) of raw goats' milk were positive for the presence of H. pylori. The study demonstrated that nested-PCR was highly sensitive for the detection of H. pylori in raw milk samples. The results of the study showed the presence of H. pylori DNA in a high percentage of both cows' and goats' raw milk samples, indicating a high possibility of inter-animal transmission within individual farms.
Název v anglickém jazyce
Prevalence and detection of Helicobacter pylori in raw cows' and goats' milk in selected farms in the Czech Republic
Popis výsledku anglicky
Helicobacter pylori is currently found in more than a half of the human population and is the cause of serious gastrointestinal diseases such as gastritis, gastric ulcers or adenocarcinoma. H. pylori infection can be transmitted to humans through contaminated raw materials and food of animal origin. In this study, individual cows' milk samples (n = 77) and individual goats' milk samples (n = 52) from two farms in Moravia (Czech Republic) were analysed. The classical culture method and the nested polymerase chain reaction (nested PCR) method were used for detection of H. pylori. The culture method did not demonstrate the presence of H. pylori in any of the examined samples. Using the nested PCR method, 31 samples (40 %) of raw cows' milk and 30 samples (58 %) of raw goats' milk were positive for the presence of H. pylori. The study demonstrated that nested-PCR was highly sensitive for the detection of H. pylori in raw milk samples. The results of the study showed the presence of H. pylori DNA in a high percentage of both cows' and goats' raw milk samples, indicating a high possibility of inter-animal transmission within individual farms.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10600 - Biological sciences
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2022
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Food and Nutrition Research
ISSN
1336-8672
e-ISSN
1338-4260
Svazek periodika
61
Číslo periodika v rámci svazku
2
Stát vydavatele periodika
SK - Slovenská republika
Počet stran výsledku
6
Strana od-do
192-197
Kód UT WoS článku
000821448700009
EID výsledku v databázi Scopus
—