Alpha mangostin inhibits proliferation, migration, and invasion of human breast cancer cells via STAT3 inhibition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F23%3A00079715" target="_blank" >RIV/00159816:_____/23:00079715 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/23:00133682

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S2667394023000035?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S2667394023000035?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.adcanc.2023.100089" target="_blank" >10.1016/j.adcanc.2023.100089</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Alpha mangostin inhibits proliferation, migration, and invasion of human breast cancer cells via STAT3 inhibition

Popis výsledku v původním jazyce

Signal Transducer and Activator of Transcription 3 (STAT3) is an identified critical protein associated with the progression of cancer. Alpha mangostin (alpha-M), a powerful dietary xanthone found to have anti-cancer properties against various cancers. However, the precise mechanism of its anti-cancer activity is not fully understood. Therefore, the current work hypothesized that targeting STAT3 with alpha-M inhibits the migration, invasion, and proliferation of breast cancer cells. Firstly, we evaluated the binding affinity of alpha-M/STAT3 complex using molecular dynamic simulations (MDS) and further we determined the likely underlying mechanism of STAT3 through in-vitro experiments. alpha-M treatment affected the levels of STAT3 phosphorylation, hnRNP-A1, PKM2, and EMT markers. alpha-M stimulation in breast cancer cells also resulted in suppressed migratory and invasive behaviour. More importantly, the treatment also affected the Ki67 and BrdU positive cells. In summary, we found the anti-migratory and anti-proliferative actions of alpha-M in breast cancer cells via STAT3 inhibition. Also, the study significantly adds a new nutraceutical for therapeutic intervention of invasive breast cancer.

Název v anglickém jazyce

Alpha mangostin inhibits proliferation, migration, and invasion of human breast cancer cells via STAT3 inhibition

Popis výsledku anglicky

Signal Transducer and Activator of Transcription 3 (STAT3) is an identified critical protein associated with the progression of cancer. Alpha mangostin (alpha-M), a powerful dietary xanthone found to have anti-cancer properties against various cancers. However, the precise mechanism of its anti-cancer activity is not fully understood. Therefore, the current work hypothesized that targeting STAT3 with alpha-M inhibits the migration, invasion, and proliferation of breast cancer cells. Firstly, we evaluated the binding affinity of alpha-M/STAT3 complex using molecular dynamic simulations (MDS) and further we determined the likely underlying mechanism of STAT3 through in-vitro experiments. alpha-M treatment affected the levels of STAT3 phosphorylation, hnRNP-A1, PKM2, and EMT markers. alpha-M stimulation in breast cancer cells also resulted in suppressed migratory and invasive behaviour. More importantly, the treatment also affected the Ki67 and BrdU positive cells. In summary, we found the anti-migratory and anti-proliferative actions of alpha-M in breast cancer cells via STAT3 inhibition. Also, the study significantly adds a new nutraceutical for therapeutic intervention of invasive breast cancer.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/LX22NPO5107" target="_blank" >LX22NPO5107: Národní ústav pro neurologický výzkum</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ADVANCES IN CANCER BIOLOGY-METASTASIS

ISSN

2667-3940

e-ISSN

2667-3940

Svazek periodika

7

Číslo periodika v rámci svazku

JUL 2023

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

100089

Kód UT WoS článku

001136029700001

EID výsledku v databázi Scopus

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