Dysregulation of butyrylcholinesterase, BCHE gene SNP rs1803274, and pro-inflammatory cytokines in occupational workers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F23%3A10458137" target="_blank" >RIV/00179906:_____/23:10458137 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62690094:18470/23:50019934

Výsledek na webu

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y69OzMScwD" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=y69OzMScwD</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.envres.2022.115195" target="_blank" >10.1016/j.envres.2022.115195</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Dysregulation of butyrylcholinesterase, BCHE gene SNP rs1803274, and pro-inflammatory cytokines in occupational workers

Popis výsledku v původním jazyce

Background.People in different occupations are exposed to a variety of xenobiotics which affect the health and physio-logical processes of the body. Butyrylcholinesterase (BChE), has been reported to play neuronal and non -neuronal roles, though its exact function is yet to be established. This study aimed to find the status and role of BChE in seven different occupational groups; gasoline fillers, auto-mechanics, carpenters, textile shop workers, furniture shop workers, electricians, and office workers.Methods.A total of 400 samples were screened. BChE activity was determined by Worek et al. method based on Ellman&apos;s principle. Pro-inflammatory cytokines were determined by ELISA. Genotypic analysis of the K-variant of BCHE gene SNP was carried out by standard molecular methods. Among seven groups, office workers were taken as a control to compare the results with all other occupational groups.Results.The results revealed a significant decrease in BChE activity in gasoline fillers (79.52%) followed by carpenters (73.49%), auto mechanics (39.76%), textile shop workers (18.07%), electricians (10.84%), and furniture shop workers (7.23%). TNF-alpha, IL-6, and IL1-13 were elevated in all groups. IL-6 and IL1-13 in gasoline fillers, and electricians were not statistically significantly increased. Binomial regression to determine the odd ratio was found to be significant (p &lt; 0.05) in all groups. However, correlation (Pearson) did not reveal significance be-tween different biochemical parameters. Genotypic analysis of the K-variant SNP of the BCHE gene showed a significant association with occupational groups when compared with control which indicates a possible asso-ciation with xenobiotics exposure and the physiological role of K-variant in understudied occupational groups.Conclusion.The study concluded that BChE and its gene SNP rs 1803274 and proinflammatory cytokines significantly dysregulates under the exposure to cumulative multiple xenobiotics in different occupational groups which may lead to pathophysiological conditions.

Název v anglickém jazyce

Dysregulation of butyrylcholinesterase, BCHE gene SNP rs1803274, and pro-inflammatory cytokines in occupational workers

Popis výsledku anglicky

Background.People in different occupations are exposed to a variety of xenobiotics which affect the health and physio-logical processes of the body. Butyrylcholinesterase (BChE), has been reported to play neuronal and non -neuronal roles, though its exact function is yet to be established. This study aimed to find the status and role of BChE in seven different occupational groups; gasoline fillers, auto-mechanics, carpenters, textile shop workers, furniture shop workers, electricians, and office workers.Methods.A total of 400 samples were screened. BChE activity was determined by Worek et al. method based on Ellman&apos;s principle. Pro-inflammatory cytokines were determined by ELISA. Genotypic analysis of the K-variant of BCHE gene SNP was carried out by standard molecular methods. Among seven groups, office workers were taken as a control to compare the results with all other occupational groups.Results.The results revealed a significant decrease in BChE activity in gasoline fillers (79.52%) followed by carpenters (73.49%), auto mechanics (39.76%), textile shop workers (18.07%), electricians (10.84%), and furniture shop workers (7.23%). TNF-alpha, IL-6, and IL1-13 were elevated in all groups. IL-6 and IL1-13 in gasoline fillers, and electricians were not statistically significantly increased. Binomial regression to determine the odd ratio was found to be significant (p &lt; 0.05) in all groups. However, correlation (Pearson) did not reveal significance be-tween different biochemical parameters. Genotypic analysis of the K-variant SNP of the BCHE gene showed a significant association with occupational groups when compared with control which indicates a possible asso-ciation with xenobiotics exposure and the physiological role of K-variant in understudied occupational groups.Conclusion.The study concluded that BChE and its gene SNP rs 1803274 and proinflammatory cytokines significantly dysregulates under the exposure to cumulative multiple xenobiotics in different occupational groups which may lead to pathophysiological conditions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Environmental Research

ISSN

0013-9351

e-ISSN

1096-0953

Svazek periodika

220

Číslo periodika v rámci svazku

MAR

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

10

Strana od-do

115195

Kód UT WoS článku

000919373200001

EID výsledku v databázi Scopus

2-s2.0-85145842315