Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear Factor ?B (NF-?B) associated proteins as putative biomarkers of metastasis in low grade breast cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F15%3A%230000614" target="_blank" >RIV/00209805:_____/15:#0000614 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.mcponline.org/cgi/pmidlookup?view=long&pmid=25903579" target="_blank" >http://www.mcponline.org/cgi/pmidlookup?view=long&pmid=25903579</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1074/mcp.M114.041335" target="_blank" >10.1074/mcp.M114.041335</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear Factor ?B (NF-?B) associated proteins as putative biomarkers of metastasis in low grade breast cancer

Popis výsledku v původním jazyce

Current prognostic factors are insufficient for precise risk-discrimination in breast cancer patients with low grade breast tumors, which, in disagreement with theoretical prognosis, occasionally form early lymph node metastasis. To identify markers forthis group of patients, we employed iTRAQ-2DLC-MS/MS proteomics to 24 lymph node positive and 24 lymph node negative grade 1 luminal A primary breast tumorsFrom the total of 4405 proteins identified (FDR<5%), the top 65 differentially expressed togetherwith 30 previously identified and control markers were analyzed also at transcript level. Increased levels of carboxypeptidase B1 (CPB1), PDZ and LIM domain protein 2 (PDLIM2), and ring finger protein 25 (RNF25) were associated specifically with lymph node positive grade 1 tumors, whereas stathmin 1 (STMN1) and thymosin beta 10 (TMSB10) associated with aggressive tumor phenotype also in high grade tumors at both protein and transcript level. For CPB1, these differences were also observed

Název v anglickém jazyce

Combined proteomics and transcriptomics identifies carboxypeptidase B1 and nuclear Factor ?B (NF-?B) associated proteins as putative biomarkers of metastasis in low grade breast cancer

Popis výsledku anglicky

Current prognostic factors are insufficient for precise risk-discrimination in breast cancer patients with low grade breast tumors, which, in disagreement with theoretical prognosis, occasionally form early lymph node metastasis. To identify markers forthis group of patients, we employed iTRAQ-2DLC-MS/MS proteomics to 24 lymph node positive and 24 lymph node negative grade 1 luminal A primary breast tumorsFrom the total of 4405 proteins identified (FDR<5%), the top 65 differentially expressed togetherwith 30 previously identified and control markers were analyzed also at transcript level. Increased levels of carboxypeptidase B1 (CPB1), PDZ and LIM domain protein 2 (PDLIM2), and ring finger protein 25 (RNF25) were associated specifically with lymph node positive grade 1 tumors, whereas stathmin 1 (STMN1) and thymosin beta 10 (TMSB10) associated with aggressive tumor phenotype also in high grade tumors at both protein and transcript level. For CPB1, these differences were also observed

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular and cellular proteomics

ISSN

1535-9476

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

17

Strana od-do

1814-1830

Kód UT WoS článku

000357434400007

EID výsledku v databázi Scopus

2-s2.0-84936806779