Transgelin is upregulated in stromal cell sof lymf node positive breast cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F16%3AN0000008" target="_blank" >RIV/00209805:_____/16:N0000008 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jprot.2015.11.025" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2015.11.025</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jprot.2015.11.025" target="_blank" >10.1016/j.jprot.2015.11.025</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Transgelin is upregulated in stromal cell sof lymf node positive breast cancer

Popis výsledku v původním jazyce

Transgelin and transgelin-2 have been discussed as potential markers of various cancers. Here we identified increased transgelin level in lymph node positive vs. negative, low grade primary breast cancer tissues using 2-DE in the cohort of 12 patients.Wefurther clinically validated 2-DE results in an independent cohort of 48 lowgrade breast cancer patients through untargeted and targeted proteomics analysis (iTRAQ-2D-LC–MS/MS, mTRAQSRM), at transcript level and using immunohistochemistry. Another group of 48 high grade tumors of different breast cancer subtypes was analyzed together with the low grade samples to test transgelin specificity for low grade tumors and to study transgelin relation to known molecular markers and histological features. The results confirmed transgelin connection with the lymph node metastasis. As a marker of a reactive tumor stroma, transgelin can be connected with the higher risk of metastasis development. Moreover, we observed signifiant down-regulation of transgelin in high vs. low grade tumors caused by decreased content of stromal cells (mainly expressing transgelin) in high grade tumor tissue. We also analyzed expression of transgelin-2 in the sekond cohort using proteomics and immunohistochemistry. Transgelin-2 was mainly expressed by epithelial cancer cells and its levels were increased in metastatic and poorly differentiated tumors. Biological significance: Both transgelin and transgelin-2 have been previously described as potential markers of many types of cancer.Weare specifying this connection to metastatic affection of lymph nodes and cell differentiation in breast cancer. In thewider context, the results of our study highlight tumor stromaas a source of cancer biomarkers and point out how measured levels of tissue markers can actually reflect cellular feature of cancer mass.

Název v anglickém jazyce

Transgelin is upregulated in stromal cell sof lymf node positive breast cancer

Popis výsledku anglicky

Transgelin and transgelin-2 have been discussed as potential markers of various cancers. Here we identified increased transgelin level in lymph node positive vs. negative, low grade primary breast cancer tissues using 2-DE in the cohort of 12 patients.Wefurther clinically validated 2-DE results in an independent cohort of 48 lowgrade breast cancer patients through untargeted and targeted proteomics analysis (iTRAQ-2D-LC–MS/MS, mTRAQSRM), at transcript level and using immunohistochemistry. Another group of 48 high grade tumors of different breast cancer subtypes was analyzed together with the low grade samples to test transgelin specificity for low grade tumors and to study transgelin relation to known molecular markers and histological features. The results confirmed transgelin connection with the lymph node metastasis. As a marker of a reactive tumor stroma, transgelin can be connected with the higher risk of metastasis development. Moreover, we observed signifiant down-regulation of transgelin in high vs. low grade tumors caused by decreased content of stromal cells (mainly expressing transgelin) in high grade tumor tissue. We also analyzed expression of transgelin-2 in the sekond cohort using proteomics and immunohistochemistry. Transgelin-2 was mainly expressed by epithelial cancer cells and its levels were increased in metastatic and poorly differentiated tumors. Biological significance: Both transgelin and transgelin-2 have been previously described as potential markers of many types of cancer.Weare specifying this connection to metastatic affection of lymph nodes and cell differentiation in breast cancer. In thewider context, the results of our study highlight tumor stromaas a source of cancer biomarkers and point out how measured levels of tissue markers can actually reflect cellular feature of cancer mass.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Proteomics

ISSN

1874-3919

e-ISSN

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Svazek periodika

132

Číslo periodika v rámci svazku

30 January 2016

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

103-111

Kód UT WoS článku

000368747400011

EID výsledku v databázi Scopus

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