MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F18%3A00078109" target="_blank" >RIV/00209805:_____/18:00078109 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/18:00069232 RIV/00216224:14740/18:00106965

Výsledek na webu

<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6285981/pdf/pone.0208580.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6285981/pdf/pone.0208580.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0208580" target="_blank" >10.1371/journal.pone.0208580</a>

Jazyk výsledku

angličtina

Název v původním jazyce

MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

Popis výsledku v původním jazyce

Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.

Název v anglickém jazyce

MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

Popis výsledku anglicky

Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30204 - Oncology

Projekt

<a href="/cs/project/NV15-34553A" target="_blank" >NV15-34553A: Využití mikroRNA v mozkomíšním moku pro diagnostiku mozkových nádorů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE [online]

ISSN

1932-6203

e-ISSN

—

Svazek periodika

13

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

"e0208580"

Kód UT WoS článku

000452640900023

EID výsledku v databázi Scopus

2-s2.0-85058123970