Application of the new insertion-deletion polymorphism kit for forensic identification and parentage testing on the Czech population

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A00009640" target="_blank" >RIV/00216208:11110/13:00009640 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11140/13:00009640 RIV/00064165:_____/13:00009640

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00414-011-0649-3" target="_blank" >http://dx.doi.org/10.1007/s00414-011-0649-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00414-011-0649-3" target="_blank" >10.1007/s00414-011-0649-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of the new insertion-deletion polymorphism kit for forensic identification and parentage testing on the Czech population

Popis výsledku v původním jazyce

We genotyped 55 unrelated Czech individuals. We also genotyped 11 trios to analyze DIPplex Kit (QIAGEN, Germany) suitability for parentage testing. DIPplex Kit contains 30 diallelic autosomal markers. INDELs in DIPplex Kit were tested with linkage disequilibrium test, which showed that they could be treated as independent markers. All 30 loci fulfill Hardy-Weinberg equilibrium. There were several significant differences between Czech and African populations, but no significant ones within European population. Probabilityof a match in the Czech population was 1 in 6.8x1012; combined power of discrimination was 99.9999999999%.Average paternity index was 1.13-1.77 for each locus;combined paternity index reached about 27,000 for a set of 30 loci. The DIPplex kit is useful as an additional panel of markers in paternity cases when mutations in STR polymorphisms are present. For application on degraded or inhibited samples, further optimization of buffer and primer concentrations is needed.

Název v anglickém jazyce

Application of the new insertion-deletion polymorphism kit for forensic identification and parentage testing on the Czech population

Popis výsledku anglicky

We genotyped 55 unrelated Czech individuals. We also genotyped 11 trios to analyze DIPplex Kit (QIAGEN, Germany) suitability for parentage testing. DIPplex Kit contains 30 diallelic autosomal markers. INDELs in DIPplex Kit were tested with linkage disequilibrium test, which showed that they could be treated as independent markers. All 30 loci fulfill Hardy-Weinberg equilibrium. There were several significant differences between Czech and African populations, but no significant ones within European population. Probabilityof a match in the Czech population was 1 in 6.8x1012; combined power of discrimination was 99.9999999999%.Average paternity index was 1.13-1.77 for each locus;combined paternity index reached about 27,000 for a set of 30 loci. The DIPplex kit is useful as an additional panel of markers in paternity cases when mutations in STR polymorphisms are present. For application on degraded or inhibited samples, further optimization of buffer and primer concentrations is needed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Legal Medicine

ISSN

0937-9827

e-ISSN

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Svazek periodika

127

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

4

Strana od-do

7-10

Kód UT WoS článku

000313164100002

EID výsledku v databázi Scopus

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