Immunomagnetic molecular probe with UHPLC-MS/MS: A promising way for reliable bronchial asthma diagnostics based on quantification of cysteinyl leukotrienes
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10189883" target="_blank" >RIV/00216208:11110/13:10189883 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388971:_____/13:00425848 RIV/60461373:22310/13:43896722 RIV/00179906:_____/13:10189883
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.jpba.2013.03.026" target="_blank" >http://dx.doi.org/10.1016/j.jpba.2013.03.026</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2013.03.026" target="_blank" >10.1016/j.jpba.2013.03.026</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Immunomagnetic molecular probe with UHPLC-MS/MS: A promising way for reliable bronchial asthma diagnostics based on quantification of cysteinyl leukotrienes
Popis výsledku v původním jazyce
A sensitive and precise method for simultaneous quantification of cysteinyl leukotrienes (=cys LTs) - leukotriene C-4 (=LTC4), leukotriene D-4 (=LTD4) and leukotriene E-4 (=LTE4) - essential biomarkers of bronchial asthma present in exhaled breath condensate (=EBC) was developed. An immunomagnetic molecular probe was prepared by anchoring cysteinyl leukotrienes antibody on the surface of functionalized monodispersed magnetic particles and used to selectively isolate cys LTs from biological matrices - EBC, plasma and urine. Immobilization and the immunoaffinity capture procedures were optimized to maximize the amount of separated cys LTs, which were detected "off-beads" after acidic elution by UHPLC-ESI-MS/MS operated in a multiple reaction monitoring mode. The developed method was characterized with high precision {= 13.6% (intra-day precision determined as RSD) and {= 14.5% (inter-day precision determined as RSD), acceptable accuracy {= 18.5% (determined as RE), and high recovery of i
Název v anglickém jazyce
Immunomagnetic molecular probe with UHPLC-MS/MS: A promising way for reliable bronchial asthma diagnostics based on quantification of cysteinyl leukotrienes
Popis výsledku anglicky
A sensitive and precise method for simultaneous quantification of cysteinyl leukotrienes (=cys LTs) - leukotriene C-4 (=LTC4), leukotriene D-4 (=LTD4) and leukotriene E-4 (=LTE4) - essential biomarkers of bronchial asthma present in exhaled breath condensate (=EBC) was developed. An immunomagnetic molecular probe was prepared by anchoring cysteinyl leukotrienes antibody on the surface of functionalized monodispersed magnetic particles and used to selectively isolate cys LTs from biological matrices - EBC, plasma and urine. Immobilization and the immunoaffinity capture procedures were optimized to maximize the amount of separated cys LTs, which were detected "off-beads" after acidic elution by UHPLC-ESI-MS/MS operated in a multiple reaction monitoring mode. The developed method was characterized with high precision {= 13.6% (intra-day precision determined as RSD) and {= 14.5% (inter-day precision determined as RSD), acceptable accuracy {= 18.5% (determined as RE), and high recovery of i
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FC - Pneumologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
e-ISSN
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Svazek periodika
81-82
Číslo periodika v rámci svazku
July-August
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
10
Strana od-do
108-117
Kód UT WoS článku
000319793500015
EID výsledku v databázi Scopus
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