In vivo visualization of cells labeled with superparamagnetic iron oxides by a sub-millisecond gradient echo sequence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10192018" target="_blank" >RIV/00216208:11110/14:10192018 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/14:00059036

Výsledek na webu

<a href="http://link.springer.com/article/10.1007%2Fs10334-013-0422-3" target="_blank" >http://link.springer.com/article/10.1007%2Fs10334-013-0422-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s10334-013-0422-3" target="_blank" >10.1007/s10334-013-0422-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vivo visualization of cells labeled with superparamagnetic iron oxides by a sub-millisecond gradient echo sequence

Popis výsledku v původním jazyce

In vivo magnetic resonance imaging (MRI) of iron-labeled pancreatic islets (PIs) transplanted into the liver is still challenging in humans. The aim of this study was to develop and evaluate a double contrast method for the detection of PIs labeled withsuperparamagnetic iron oxide (SPIO) nanoparticles. Materials and methods: A double-echo three-dimensional (3D) spoiled gradient echo sequence was adapted to yield a sub-millisecond first echo time using variable echo times and highly asymmetric Cartesianreadout. Positive contrast was achieved by conventional and relative image subtraction. Experiments for cell detection efficiency were performed in vitro on gelatin phantoms, in vivo on a Lewis rat and on a patient 6 months after PI transplantation. Results: It was demonstrated that the proposed method can be used for the detection of transplanted PIs with positive contrast in vitro and in vivo. For all experiments, relative subtraction yielded comparable and in some cases better contra

Název v anglickém jazyce

In vivo visualization of cells labeled with superparamagnetic iron oxides by a sub-millisecond gradient echo sequence

Popis výsledku anglicky

In vivo magnetic resonance imaging (MRI) of iron-labeled pancreatic islets (PIs) transplanted into the liver is still challenging in humans. The aim of this study was to develop and evaluate a double contrast method for the detection of PIs labeled withsuperparamagnetic iron oxide (SPIO) nanoparticles. Materials and methods: A double-echo three-dimensional (3D) spoiled gradient echo sequence was adapted to yield a sub-millisecond first echo time using variable echo times and highly asymmetric Cartesianreadout. Positive contrast was achieved by conventional and relative image subtraction. Experiments for cell detection efficiency were performed in vitro on gelatin phantoms, in vivo on a Lewis rat and on a patient 6 months after PI transplantation. Results: It was demonstrated that the proposed method can be used for the detection of transplanted PIs with positive contrast in vitro and in vivo. For all experiments, relative subtraction yielded comparable and in some cases better contra

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/NT13099" target="_blank" >NT13099: Optimalizace kultivačních podmínek izolovaných Langerhnasových ostrůvků určených k transplantaci</a><br>

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Magnetic Resonance Materials in Physics, Biology, and Medicine

ISSN

0968-5243

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

329-337

Kód UT WoS článku

000339833300006

EID výsledku v databázi Scopus

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